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Invitrogen
FceR1 alpha Monoclonal Antibody (MAR-1), PE-eFluor™ 610, eBioscience™
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FIGURE: 1 / 14
FceR1 alpha Antibody (61-5898-82) in Flow
Staining of mouse bone marrow-derived mast cells with 0.125 µg of Armenian Hamster IgG Isotype Control PE-eFluor® 610 (Product # 61-4888-82) (blue histogram) or 0.125 µg of Anti-Mouse Fc epsilon Receptor I alpha (FceR1) PE-eFluor® 610 (purple histogram). Total viable cells as determined by Fixable Viability Dye eFluor® 450 (Product # 65-0863-14), were used for analysis.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
61-5898-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Human,
Mouse
Host/Isotype
Armenian hamster
/ IgG
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
MAR-1
Conjugate
PE-eFluor™ 610
PE-eFluor™ 610
PE-eFluor™ 610
Excitation/Emission Max
565/606 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The MAR-1 monoclonal antibody reacts with the Fc epsilon Receptor I alpha subunit, an IgE-binding subunit lacking signal-transducing ability. Fc epsilon RI alpha is expressed on mast and basophil cells and is up-regulated by the presence of IgE. Fc epsilon RI alpha forms a tetrameric complex with one beta and two gamma subunits. The beta and gamma subunits possess immunoreceptor tyrosine-based activation motifs (ITAM). The Fc epsilon RI complex plays an important role in triggering IgE-mediated allergic reactions.
Applications Reported: This MAR-1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This MAR-1 antibody has been tested by flow cytometric analysis of mouse bone marrow-derived mast cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Fc epsilon RI alpha, also known as FceR1 alpha, is a subunit of the high-affinity receptor for IgE, primarily expressed on mast cells and basophils. Its expression is upregulated by the presence of IgE. Fc epsilon RI alpha forms a tetrameric complex with one beta and two gamma subunits, which are essential for signal transduction. The beta and gamma subunits contain immunoreceptor tyrosine-based activation motifs (ITAMs). The Fc epsilon RI complex plays a crucial role in triggering IgE-mediated allergic reactions. When allergen-bound IgE molecules bring together two or more high-affinity IgE receptors, it leads to the release of mediators such as histamine, which are responsible for allergy symptoms. This process couples allergens and mast cells to initiate inflammatory responses characteristic of allergic disorders like hay fever and asthma. The release of histamine and proteases also leads to the synthesis of prostaglandins and leukotrienes, which are potent effectors of the hypersensitivity response.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: fc epsilon r1; fc-epsilon RI-alpha; high affinity IgE receptor alpha subunit precursor; igE Fc receptor subunit alpha; RP11-550P17.3
Gene Aliases: Fce1a; fcepsilonri; FcERI; Fcr-5
UniProt ID: (Mouse) P20489
Entrez Gene ID: (Mouse) 14125
positive regulation of type I hypersensitivity
serotonin secretion
signal transduction
cell surface receptor signaling pathway
immunoglobulin mediated immune response
type I hypersensitivity
leukotriene biosynthetic process
positive regulation of granulocyte macrophage colony-stimulating factor production
positive regulation of interleukin-3 production
Fc-epsilon receptor signaling pathway
type 2 immune response
mast cell degranulation
positive regulation of mast cell degranulation
eosinophil degranulation
positive regulation of MAP kinase activity
positive regulation of JUN kinase activity
positive regulation of peptidyl-tyrosine phosphorylation
regulation of immune response
positive regulation of calcium-mediated signaling
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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