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CD117 (c-Kit) Monoclonal Antibody (104D2), Super Bright™ 600, eBioscience™
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FIGURE: 1 / 6
CD117 (c-Kit) Antibody (63-1178-42) in Flow
Staining of normal human peripheral blood cells with the Human Hematopoietic Lineage eFluor® 450 Cocktail (Product # 22-7775-72), Anti-Human CD34 FITC (Product # 11-0349-42) and Mouse IgG1 K Isotype Control Super Bright 600 (Product # 63-4714-82) (left) or Anti-Human CD117 (c-Kit) Super Bright 600 (right). Lineage negative/low cells were used for analysis.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
63-1178-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
104D2
Conjugate
Super Bright™ 600
Super Bright™ 600
Super Bright™ 600
Excitation/Emission Max
414/601 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The 104D2 monoclonal antibody reacts with human CD117, also known as c-Kit, Steel factor receptor, and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145 kDa molecule is expressed by hematopoietic progenitor cell subsets and mast cells. The interaction of c-Kit and Steel factor promotes proliferation and differentiation of hematopoietic progenitor cells and mast cell differentiation. CD117 is also expressed by melanocytes and plays a role in signaling and activation of these cells.
This 104D2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401-42) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 2-8°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorochrome performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 405 nm; Emission: 600 nm; Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
KIT (c-KIT) is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). KIT was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT together with its ligand regulates growth and activation of a variety of hemopoietic and non-hemopoietic cells. Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Recently, deregulation of the KIT receptor TK by the prevalent activation loop mutation D816V has served as a focal point in therapeutic strategies aimed at curbing neoplastic mast cell growth. c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells and gastrointestinal tract cajal cells. Upon binding of its ligand stem cell factor (SCF), c-kit dimerizes, resulting in receptor activation and autophosphorylation of various tyrosine residues including tyrosine 703 located on the cytoplasmic domain of the receptor. This modification allows docking of Grb2 and activation of the Ras/ERK signaling pathway. SCF/c-kit can activate multiple downstream signaling pathways including PI3K, PLC-gamma and JAK/STAT. c-kit receptor activation is essential for hematopoiesis, stem cell maintenance and gametogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: c-Kit protooncogene; ckit; p145 c-kit; piebald trait protein; protein kinase; proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; tyrosine-protein kinase Kit; unnamed protein product; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein
Gene Aliases: C-Kit; CD117; MASTC; PBT; SCFR
UniProt ID: (Human) P10721
Entrez Gene ID: (Human) 3815
ovarian follicle development
hematopoietic progenitor cell differentiation
immature B cell differentiation
mast cell chemotaxis
positive regulation of cytokine production involved in immune response
positive regulation of dendritic cell cytokine production
chemotaxis
inflammatory response
signal transduction
transmembrane receptor protein tyrosine kinase signaling pathway
spermatogenesis
positive regulation of cell proliferation
germ cell migration
regulation of cell shape
gonad development
visual learning
male gonad development
cell migration
cytokine-mediated signaling pathway
stem cell population maintenance
lamellipodium assembly
actin cytoskeleton organization
hemopoiesis
B cell differentiation
T cell differentiation
erythrocyte differentiation
melanocyte differentiation
positive regulation of cell migration
positive regulation of pseudopodium assembly
positive regulation of mast cell cytokine production
somatic stem cell population maintenance
embryonic hemopoiesis
megakaryocyte development
Fc receptor signaling pathway
Kit signaling pathway
erythropoietin-mediated signaling pathway
regulation of cell proliferation
positive regulation of tyrosine phosphorylation of STAT protein
mast cell degranulation
positive regulation of MAPK cascade
pigmentation
tongue development
leukocyte activation
positive regulation of Notch signaling pathway
positive regulation of JAK-STAT cascade
response to cadmium ion
protein autophosphorylation
positive regulation of long-term neuronal synaptic plasticity
digestive tract development
stem cell differentiation
epithelial cell proliferation
regulation of developmental process
detection of mechanical stimulus involved in sensory perception of sound
positive regulation of protein kinase B signaling
cell chemotaxis
mast cell differentiation
mast cell proliferation
melanocyte migration
melanocyte adhesion
positive regulation of pyloric antrum smooth muscle contraction
positive regulation of colon smooth muscle contraction
positive regulation of small intestine smooth muscle contraction
positive regulation of vascular smooth muscle cell differentiation
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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