Expi293™ Expression Medium

Name: Expi293™ Expression Medium
SKU: A1435104

Expi293 Expression Medium designed for growth and transfection of suspension-adapted human embryonic kidney (HEK) 293 cells.

Change view

Catalog Number	Quantity
A1435104	20 L
A1435103	10 L
A1435101	1 L
A1435102	6 x 1 L

4 Options

Catalog number A1435104

Price (MXN)

Quantity:

20 L

Expi293 Expression Medium is a chemically defined, serum-free, protein-free medium for growth and transfection of suspension-adapted human embryonic kidney (HEK) 293 cells. It is specifically designed as a core component to support high density culture of Expi293F cells in the Expi293 Expression System for scalable transient protein expression.

Supports growth of suspension 293 cultures to densities of over 1.5 x 10^7 cells/mL
Transfection compatible serum-free suspension culture medium enables transfection efficiencies of greater than 70% using ExpiFectamine transfection reagent
Enables sustained, high level expression of high density transiently transfected cultures, achieving yields of up to 1 gram per liter of recombinant protein
Supports growth and transfection of 293 cells in culture formats of less than 1 mL in multiwell plates to greater than 10 L in disposable bioreactors
Does not contain phenol red

Expi293 Expression Medium is formulated with GlutaMAX-I reagent. It is ready to use, with no supplementation required. The chemically defined formulation results in high reproducibility and lot-to-lot reliability. Expi293 Expression Medium contains no human or animal-origin components. The medium is not recommended for adherent cell cultures.

For Research use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

Specifications

Cell LineExpi293F™

Product LineExpi293

Product TypeExpression Medium

Quantity20 L

ClassificationAnimal Origin-free, Chemically-defined, Protein-free, Serum-free

FormLiquid

With AdditivesGlutaMAX

Without AdditivesNo Phenol Red

Unit SizeEach

Contents & Storage

One 20L Universal Bag
Store in the dark at 2-8°C.

Frequently asked questions (FAQs)

Can I purify my protein from Expi293 Expression Medium using ProBond or Ni-NTA purification systems?

Expi293 Expression Medium is not directly compatible with ProBond or Ni-NTA purification systems. We recommend performing a buffer exchange or dialyzing the samples before His-tag purification.

Can I use a different 293 cell line with the Expi293 Expression System?

Other 293 cell lines may be used with the Expi293 Expression System. However, before these cell lines may be used for transfection studies, they must be adapted to serum-free, suspension culture in Expi293 Expression Medium and evaluated for transfection and expression.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

Which vector do you recommend using with the Expi293 Expression System?

We recommend using the pcDNA 3.4-TOPO TA vector (Cat. No. A14697). This vector contains the native, full-length CMV promoter and a WPRE (Woodchuck Posttranscriptional Regulatory Element) downstream of the cloning site, both of which contribute to high level gene expression (about 2-3 fold higher expression than with pcDNA3.3 TOPO vector, which in turn provides 2-5 fold higher expression than with a standard pcDNA vector). Of course, the expression level is also protein-dependent.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

Is the Expi293 Expression System completely animal-origin free (AOF)?

All the components of the system are animal-origin free except for the Opti-MEM I Reduced Serum Medium that is serum-free but not animal-origin free. Please see the Application Note for using the Expi293 Expression System under animal origin-free conditions:
http://www.thermofisher.com/content/dam/LifeTech/migration/files/proteins-expression-isolation-analysis/pdfs.par.5943.file.dat/expi-293-animal-origin-free-co25751.pdf

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

I am interested in a mammalian expression system that will allow me to obtain very high yields of my protein. What kind of systems do you offer?

For stable high-yield expression, we offer the Freedom CHO-S and Freedom DG44 kits. For transient high-yield expression, we offer the ExpiCHO Expression System, Expi293 Expression System, FreeStyle 293 Expression System, FreeStyle Max 293 Expression System, and FreeStyle Max CHO Expression System. For high-yield expression of functional membrane proteins in Exp293F cells, we offer the Expi293 MembranePro Expression System (Cat. Nos. A25869, A25870) that combines the scalability and ease of use of Expi293 and the technology of MembranePro to allow an increase of more than 20-fold in membrane protein yield compared to the standard, adherent culture MembranePro Functional Protein Expression System. Please see the Application Note (http://www.thermofisher.com/content/dam/LifeTech/global/life-sciences/ProteinExpressionAnalysis/pdfs/PG1391-PJ6257-CO28704-Expi293Adaption-MembraneProTech_AppNoteGlobal-FHR.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

View all Expi293™ Expression Medium FAQs