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Gibco™

Expi293™ Expression System Kit

Catalog number: A14635
Gibco™

Expi293™ Expression System Kit

Catalog number: A14635
Catalog Number
A14635
Unit Size
1 kit
Price (USD)
2,270.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A146351 kit
2,270.00
Your Price:
***
Product Overview
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The Expi293 Expression System is a complete solution for rapid, high-yield protein production from mammalian 293 cells. It is based on integrated components including high-density Expi293F cells in Expi293 Expression Medium and the cationic lipid-based ExpiFectamine 293 transfection reagent in combination with specialized transfection enhancers.

The Expi293 Expression System features:
• Up to 1 g/L of protein; 2- to 10-fold higher protein yields than previous generation transient expression systems such as the FreeStyle 293 Expression System
• Rapid production of recombinant proteins in 5–7 days
• High density cultures of Expi293F cells that result in more expressing cells per milliliter of culture
• Native folding and mammalian post-translational modifications of expressed proteins
• Easy, robust culture and transfection protocols
• Scalable expression from <1 mL to >10 L

The components included in the Expi293 Expression System Kit are: two vials of frozen Expi293F cells, one liter of Expi293 Expression Medium, one ExpiFectamine 293 Transfection kit sufficient to transfect 1 liter of culture, Opti-MEM I Reduced Serum Medium, and an antibody-expressing positive control vector. All components of the Expi293 Expression System are also available for individual purchase.

Engineered Expi293F cell lines for a range of applications are also available for individual purchase or as part of kits: Expi293F GnTI- Cells (Cat. No. A39240). Expi293F Inducible Cells (Cat. No. A39241), and Expi293F Inducible GnTI- Cells (Cat. No. A39242).

For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Application)
Protein Expression
No. of Reactions
33 Reactions
Product Type
Expression System Kit
Quantity
33 Reactions
Expression System
Mammalian
Yield
mg to g quantities of protein
Promoter
CMV
Delivery Type
Transfection
Cell Line
Expi293F™
Expression Stability
Transient
Key Functions
Protein Production, Constitutive Expression
Protein Tag
Untagged
System Type
Expi293™ Expression System
Product Line
Expi293™
Transfection Technique
Lipid-Based Transfection
Cell Type
293-derived cells

Contents & Storage

2 vials frozen Expi293F cells
1 bottle 1000 mL Expi293 Expression Medium
1 ExpiFectamine 293 transfection kit for 1 L of culture
1 vial Antibody Expressing Positive Control Vector
1 bottle 100 mL Opti-MEM Reduced Serum Medium

Store all components at +2°C to +8°C, except frozen cells are stored in liquid nitrogen

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