Expi293™ Expression System Kit

Catalog number: A14635

Gibco™  Related applications: Antibody & Cell Culture Production | Protein Expression | Transfection

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Description

The Expi293™ Expression System is a major advance in transient expression technology for rapid and ultra high-yield protein production from mammalian cells. It is based on high density culture of Expi293F™ cells in Expi293™ Expression Medium. Transient expression is powered by the cationic lipid-based ExpiFectamine™ 293 transfection reagent in combination with specialized transfection enhancers. All components work in concert to generate 2- to 10-fold higher protein yields than previous generation transient expression systems such as the FreeStyle™ 293 Expression System. Expression yields of >1 gram per liter of transfected culture have been demonstrated for some antibody and non-antibody proteins.

The Expi293™ Expression System provides:

Rapid production of milligram to gram quantities of recombinant protein in mammalian cells
High density cultures of Expi293F™ cells that result in more expressing cells per milliliter of culture and higher protein yields
Native folding and mammalian post-translational modifications, such as glycosylation, of expressed proteins
Easy, robust culture and transfection protocols that readily fit into current transient expression workflows
Scalable expression that can be applied to cultures from<1 mL to >10 L, thus generating the amount of protein needed

The components included in the Expi293™ Expression System Kit are: 2 vials of frozen Expi293F™ cells, 1 liter of Expi293™ Expression medium, one ExpiFectamine™ 293 transfection kit sufficient to transfect 1 liter of culture, Opti-MEM® reduced serum media, and an antibody-expressing positive control vector. All components of the Expi293™ Expression System are also available for purchase separately.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Line: Expi293F™
Delivery Method: Transfection
Expression: Mammalian
Expression Stability: Transient
Key Function: Constitutive Expression, Protein Production
Number of Reactions: 33 Reactions
Product Size: 1 kit
Promoter: CMV
Protein Tag or Fusion: Untagged
System: Expi293™ Expression System

Contents & storage

2 vials frozen Expi293F™ cells
1 bottle 1000 mL Expi293™ Expression Medium
1 ExpiFectamine™ 293 transfection kit for 1 L of culture
1 vial Antibody Expressing Positive Control Vector
1 bottle 100 mL Opti-MEM°C Reduced Serum Medium

Store all components at +2°C to +8°C, except frozen cells are stored in liquid nitrogen

Documents

Manuals & protocols

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