Gibco™

rhLaminin-521

Catalog number: A29249
Gibco™

rhLaminin-521

Catalog number: A29249
Catalog Number
A29249
Unit Size
1 mg
Price (USD)
796.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
100 µg
Price (USD)
86.75
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A292491 mg
796.00
Your Price:
***
A29248100 µg
86.75
Your Price:
***
Product Overview
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Additional Information
Recommendations
Recombinant human Laminin 521 (rhLaminin-521) is a recombinant human protein that provides a defined surface for feeder-free culture of human pluripotent stem cells (PSCs). When used with Essential 8™ Medium, rhLaminin-521 has been proven to promote cellular survival under stressful conditions in the absence of small molecule inhibitors. rhLaminin-521 has been shown to support PSC growth for >20 passages without any signs of karyotypic abnormalities and to maintain the ability of PSCs to differentiate into all three germ line lineages. rhLaminin-521 also provides optimum reprogramming efficiency of human somatic cells as well as human pluripotent stem cell survival during feeder-dependent to feeder-free transitions when used in conjunction with Essential 8 Medium. For a kit that combines the medium and substrate, see the Essential 8™ Adaptation Kit.

rhLaminin-521 is:
• Robust—transition cells between feeder-dependent and feeder-free culture systems with minimal effort
• Efficient—easily reprogram human fibroblasts to pluripotent stem cells
• Physiologically relevant—expand cells with minimal differentiation
• Scalable—passage stem cell lines as single cells with no impact to genetic stability

Trouble-free transition
When used with Essential 8 Medium, rhLaminin-521 provides a robust culture matrix that supports pluripotent stem cell adherence and expansion during the transition from feeder-dependent culture systems.

Efficient reprogramming
rhLaminin-521 has been shown to increase the number of colonies obtained during reprogramming of human fibroblasts, which can be important for in vitro disease modeling.

Confident expansion
rhLaminin-521 is expressed in blastocysts and therefore provides an environment mimicking the human stem cell niche.

Easy scale-up
rhLaminin-521 promotes stable pluripotent stem cell maintenance as single cells in the absence of ROCK inhibitors.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Source
Xeno Free
Classification
Xeno-Free
pH
6 to 8
Product Type
Supplement
Purity or Quality Grade
hESC-Qualified
Shipping Condition
Dry Ice
Quantity
1mg
Cell Type
Stem Cell

Contents & Storage

Store in freezer (-10 to -30°C)

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