Applied Biosystems™

TaqMan™ Advanced miRNA Human Serum/Plasma 96-well Plates, fast

Catalog number:  A31878

Related applications:

Real Time PCR (qPCR)

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Description

The Applied Biosystems TaqMan Advanced Serum/Plasma 96-Well Plates, Fast, provide the convenience of pre-spotted TaqMan Advanced miRNA assays in a 96-well fast plate format. This product contains a set of 2 plates for profiling of up to 188 unique miRNAs in serum/plasma samples, and includes endogenous and exogenous miRNA controls for normalization of data results.

Features of the TaqMan Advanced Serum/Plasma 96-Well Plates include:
Reproducibility—pre-spotted assays are designed to reduce pipetting error and minimize experimental variability
Sample savings—requires only 2 μL of purified plasma/serum or 1–10 ng of total RNA per sample
Updated content—assay content updated for mirBase v.21
Easy to use—just add a mix of diluted miR-Amp and master mix and run the qPCR; no additional pipetting required
Gold-standard TaqMan qPCR chemistry—TaqMan Advanced miRNA assays draw on Thermo Fisher Scientific's industry-leading bioinformatics assay design pipeline and verification system to help ensure high specificity and minimal cross-reactivity, even for closely-related miRNA families

Product details:
Number of plates: 2
Number of unique miRNAs (not including exogenous controls): 188
Exogenous controls: cel-miR-39-3p, ath-miR159a
Endogenous control: hsa-miR-16-5p
Compatible with the following Applied Biosystems qPCR instruments: Viia7, StepOnePlus, 7500/7500 Fast, QuantStudio 3, QuantStudio 5, QuantStudio 6, QuantStudio 7 Flex, and QuantStudio 12k Flex
Recommended master mix: TaqMan Fast Advanced Master Mix

Notes
• TaqMan Advanced miRNA plates require the use of the TaqMan Advanced cDNA Synthesis Kit, which uses a universal reverse transcription (RT) chemistry to prepare the cDNA template for use with TaqMan Advanced miRNA assays for detection and quantification of mature human miRNAs in biological samples.
• These plates are not compatible with the first-generation TaqMan MicroRNA cDNA Synthesis Kit, which uses an miRNA-specific reverse transcription chemistry.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method: Primer-Probe Detection
For Use With (Equipment): 7500 Fast System,7900HT Fast System,QuantStudio™ 12k Flex,QuantStudio™ 3,QuantStudio™ 5,QuantStudio™ 6 Flex,QuantStudio™ 7,StepOnePlus™ System,ViiA™ 7 System
PCR Method: 2-Step qRT-PCR
Product Line: TaqMan™
Product Size: 2 plates
Reaction Speed: Fast
Sample Type (General): Blood,Cells,FFPE & Fixed Samples,Liquid Samples (e.g. Serum),Tissue
Shipping Condition: Room Temperature

Contents & storage

Store at room temperature.