Invitrogen™

TrueCut™ Cas9 Protein v2

Catalog number:  A36498

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}} also known as {{product.formattedSku}} 
{{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

Invitrogen TrueCut Cas9 Protein v2 is a next-generation CRISPR Cas9 protein engineered to deliver maximum editing efficiency. Features include:

• Consistently high editing efficiency in all tested cell lines including standard, immune, primary, and stem, with up to 2X higher editing efficiency in difficult targets than the competition
• High quality—manufactured under strict ISO 13485 quality standards
• Validated protocols for a large number of cell types help you achieve success faster

TrueCut Cas9 Protein v2 is recombinant Streptococcus pyogenes Cas9 (wt) protein, purified from E. coli, for genome editing with CRISPR technology. Cas9 protein forms a very stable ribonucleoprotein (RNP) complex with the guide RNA (gRNA) component of the CRISPR/Cas9 system. Incorporation of nuclear localization signals (NLS) aids delivery to the nucleus, increasing the rate of genomic DNA cleavage.

• Transfection-ready, using either lipid-mediated transfection reagents or electroporation
• Eliminates time-consuming cloning steps
• Available in 2 concentrations:
    --1 μg/μL for use in standard editing scenarios
    --5 μg/μL for optimization of editing conditions in more difficult scenarios such as in primary or embryonic cells, microinjection, or when screening multiple gRNA sequences at a time
    --For custom sizes or concentrations, please inquire

Options for obtaining CRISPR gRNA for use with TrueCut Cas9 Protein v2:
1. Order transfection-ready TrueGuide synthetic gRNAs
2. Generate transfection-ready gRNA in as little as four hours including template assembly with our GeneArt Precision gRNA Synthesis Kit
3. Have our Custom Services team design, synthesize, and purify in vitro (IVT) gRNA sequences for you

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Research Category: Genome Editing
Accession Number: NC_002737.2:854751-858857
Expression: E. coli
Gene Aliases: cas5, csn1
Gene ID: 901176
Gene Symbol: Spy_1046
Product Line: Invitrogen™
Product Size: 100 µg
Protein Form: Recombinant
Protein Tag or Fusion: Untagged
Purity or Quality Grade: Molecular Biology Grade
Shipping Condition: Dry Ice
Validated Application: Genome Editing

Contents & storage

20 µL TrueCut Cas9 Protein v2 (5 mg/mL)
Store at -5 to -30°C.

Documents

Manuals & protocols