Pierce™ Alkylating Reagents

Name: Pierce™ Alkylating Reagents
SKU: A39271

Reactivos alquilantes proporcionados en formatos de un solo uso y pesados previamente para agilizar los flujos de trabajo de preparación de proteínas.

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Número de catálogo	Cantidad	Incluye
A39271	30 x 9,3mg	Yodoacetamida, formato No-Weigh™
A39270	10 x 2mg	Cloroacetamida, formato No-Weigh™

2 opciones

Número de catálogo A39271

Precio (MXN)

Cantidad:

30 x 9,3mg

Incluye:

Yodoacetamida, formato No-Weigh™

La yodoacetamida o la cloroacetamida Thermo Scientific Pierce se suministran en un formato No-Weigh, en el que pequeñas cantidades de reactivo se pesan previamente y se envasan para un solo uso. Este formato de un solo uso evita la contaminación y la pérdida de reactividad del reactivo a lo largo del tiempo asociados con la apertura y el cierre repetitivos de un solo vial. El formato permite el uso de un vial de reactivo fresco cada vez y elimina las molestias de pesar pequeñas cantidades de reactivo.

Los reactivos de alquilación de yodoacetamida y cloracetamida son reactivos alquilantes reactivos a sulfhidrilo que se utilizan para bloquear la reducción de los residuos de cisteína para la caracterización de proteínas y el mapeo de péptidos. La alquilación con yodoacetamina o cloroacetamida tras la reducción de la cisteína resulta en la adición covalente de un grupo de carbamidometilo (57,07 Da) y previene la formación de enlaces de disulfuro. Los agentes reductores añadidos después de la alquilación reaccionarán con el exceso de iodoacetamida.

For Research Use Only. Not for use in diagnostic procedures.

Especificaciones

IncluyeYodoacetamida, formato No-Weigh™

Línea de productosPierce

Cantidad30 x 9,3mg

Tipo de productoReactivo alquilante

Unit SizeEach

Preguntas frecuentes

What is the purity level for Pierce Iodoacetamide (Cat. No. A39271)?

The purity of Pierce Iodoacetamide, measured by NMR, is 99%.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How does a product with "No-Weigh" format reduce my concern over reagent stability?

The No-Weigh product format, where small amounts of reagent are pre-weighed and packaged separately, prevents the contamination and loss of reagent reactivity over time associated with repetitive opening and closing of a single vial. This format enables use of a fresh vial of reagent each time and eliminates the hassle of weighing out small amounts of reagent. Please view additional information and the video at: https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-labeling-crosslinking/no-weigh-packaging.html

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Citations & References (2)

Citations & References

Abstract

Clean and Complete Protein Digestion with an Autolysis Resistant Trypsin for Peptide Mapping.

Authors:Muriithi B,Ippoliti S,Finny A,Addepalli B,Lauber M

Journal:Journal of proteome research

PubMed ID:39392678

Peptide mapping requires cleavage of proteins in a predictable fashion so that target protein-specific peptides can be reliably identified and quantified. Trypsin, a commonly used protease in this process, can also undergo self-cleavage or autolysis, thereby reducing the effectivity and even cleavage specificity at lysine and arginine residues. Here, we ... More

Proximity-CLIP and Expedited Non-Radioactive Library Preparation of Small RNA Footprints for Next-Generation Sequencing.

Authors:Anastasakis D,Benhalevy D,Hafner M

Journal:Current protocols in molecular biology

PubMed ID:32438484

During the course of their life cycle, most RNAs move between several cellular environments where they associate with different RNA binding proteins (RBPs). Reciprocally, a significant portion of RBPs reside in more than a single cellular compartment, where they can interact with discrete RNAs and even exert distinct biological roles. ... More