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View additional product information for KingFisher™ Duo Prime Purification System with SmartStart - FAQs (A39956)
11 product FAQs found
The User Manual for the BindIt Software can be downloaded from the BindIt Software product page online, under the Documents & Downloads section. It is also included within the BindIt Software installation file under "Documentation".
Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.
Yes we have an application note that demonstrates the lethal effect of UVC irradiation on bacterial growth and deactivation of human gDNA, "Decontamination of the KingFisher Duo Prime processing chamber using UV light".
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You will receive the following tip heads when you purchase a KingFisher Duo Prime instrument:
• 6-tip head (for 24 deep-well plate)
• 12-tip head (for 96 deep-well plate)
Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.
We do not sell elution strips for the KingFisher Duo Prime 24 Deep-Well format. A 24 Deep-Well plate must be used for elution with the 24 Deep-Well protocol.
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Yes, the KingFisher Duo Prime barcode reader is able to read the 2D barcode on the Matrix 500 µL ScrewTop Tubes. The barcode reader can generate an error if the tubes are in the tube rack and the barcode position is not able to be scanned. If this occurs, we recommend scanning each tube individually.
Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.
You can only run two protocols on the KingFisher Duo Prime if the following criteria are met:
• Both scripts only use one plate
• Both scripts are using the 96-well format
The 24-well plate format always requires two plates, so it cannot run two protocols. If each script uses one plate and it is the 96-well format, you can run two protocols using the following instructions:
1. Start the first protocol as normal.
2. Once the first protocol has started, scroll back over to the protocol selection tab and select the second script. The second script can be the same as the first script or a different script.
3. Start the second protocol.
4. Select "Start" and load the second plate.
The instrument will be processing the first plate while you start the second plate. When the first plate is done, the KingFisher Duo Prime instrument will automatically start processing the second plate.
Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.
Please follow these steps to load a script from your computer to the KingFisher Flex or KingFisher Duo/Duo Prime instrument:
1. Use the USB cable to connect the PC to the instrument.
2. Turn on the instrument.
3. Open the BindIt software and click the Connect button.
4. Select "Find New Instrument." BindIt will automatically connect to the instrument and the instrument screen will say "Computer Control".
5. Click the "Transfer" Button.
6. Click the "Upload" button.
7. Browse to find the file and double-click it.
8. In the new window that opens:
a. KingFisher Flex: you will be prompted to enter a new name – this is not necessary if the name only contains letters, digits, dash or underscore.
• If the name contains spaces, apostrophes or any other character, you should enter a new name containing only letters, digits, dash (-) or underscore (_).
• Click the "Category" pulldown to select the instrument folder you want to load the script to.
• Click OK.
b. KingFisher Duo/Duo Prime: The KingFisher Duo and Duo Prime instruments accept all characters in the filename.
• Click the "Category" pulldown to select the instrument folder you want to load the script to.
• Click OK.
Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.
The KingFisher Flex and KingFisher Duo instruments no longer come with pre-installed scripts. If your instrument begins with the following serial numbers, it will not have pre-loaded scripts:
• KingFisher Flex serial number 711-1951 or greater
• KingFisher Duo Prime serial number 706-1366 or greater
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No training is provided with the purchase of a KingFisher Flex or KingFisher Duo Prime instrument, but you can purchase training separately. We offer onsite half-day and full-day trainings for the KingFisher Flex and KingFisher Duo Prime instruments, depending on your needs. Please contact your local instrument sales representative to obtain a quote. The training will be led by your regional Field Application Scientist.
Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.
Scripts for processing large volumes of whole blood (up to 1 mL) can be found on the MagMAX Multi-Sample Ultra kit product page under the Product Literature section at the bottom of the page. There are two scripts available:
• KingFisher DUO file: A25597_Large_Volume_Blood.bdz
• KingFisher Flex file: A25597_Large_Volume_Blood.bdz
*Use the page scrolling function to see the complete list of product literature for this product.
Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.
The KingFisher Duo Purification System consumables are listed on the following website and they vary depending on whether you are using the 6- or 12-pin magnet: https://www.thermofisher.com/order/catalog/product/97003500