Verso 1-step RT-qPCR Kit, with separate ROX vial

Catalog number: AB4100C

Thermo Scientific™  Related applications: Real Time PCR (qPCR)

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The Thermo Scientific Verso 1-step RT-qPCR Kit for probe chemistry offers a quick and simple way to detect mRNA. The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures. This version of the kit comes with a separate vial of ROX reference dye.

Features of the Verso 1-step RT-qPCR Kit:

• Eliminate the need for DNase treatments—RT Enhancer (optional) prevents gDNA carry-over
• RT and qPCR steps performed in single tube—convenience and reduced contamination risk
• Use of the entire RT reaction as template for qPCR—improved sensitivity
• Verso RT enzyme is highly sensitive with broad dynamic range—accurate detection of RNA from 1 pg-1 ug
• Verso RT enzyme has a wide working temperature range (42 to 57°C)—improved success with GC-rich and other difficult templates
• Contains an inert blue dye for ease of pipetting during reaction set up

An optional RT Enhancer is also provided and can be used during the RT step to remove any DNA contamination, eliminating the need for DNase I treatment. All Verso 1-step RT-qPCR kits incorporate an inert dye to significantly enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof.

In a 1-step approach to RT-qPCR, both the RT step and qPCR steps are performed in a single tube (1-step RT-qPCR). Adopting a 1-step approach is ideal when no cDNA storage is necessary for subsequent experiments. The approach minimizes the risk of contamination and improves reproducibility by reducing sample handling.


• Gene expression analysis
• RNAi validation
• Microarray validation
• Pathogen detection
• Genetic testing
• Disease research

Related Products
Verso 1-step RT-qPCR Mix, low ROX
Verso 1-step RT-qPCR Mix, ROX
Verso 1-step RT-qPCR Kit, with separate ROX vial
For Research Use Only. Not for use in diagnostic procedures.


PCR Method: 1-Step qRT-PCR
Detection Method: Primer-Probe Detection
For Use With (Equipment): Cepheid SmartCycler, MJ Chromo4, Corbett RotorGene, Bio-Rad CFX384 Touch™, Eppendorf MasterCycler RealPlex, Roche LightCycler 480, BioRad CFX96™, MJ Opticon, BioRad MyiQ, BioRad iCycler iQ, BioRad iQ5
Sample Type (General): RNA
Product Size: 400 reactions

Contents & storage

•1 x 100 µL Verso Enzyme Mix
•1 x 500 µL RT enhancer
•1 x 5 mL 2X 1-Step qPCR Mix
•1 x 25µL ROX, 1mM
Sufficient for 400 x 25 µL reactions.

Store at -20°C


Manuals & protocols