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Thermo Scientific™

Verso 1-step RT-qPCR Kit, SYBR Green, low ROX

The Thermo Scientific Verso 1-step RT-qPCR Low ROX Kit for SYBR Green chemistry offers a quick and simple way to자세히 알아보기
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카탈로그 번호반응 수
AB4106C
AB-4106/C으로도 사용됨
384 Reactions
AB4106A
AB-4106/A으로도 사용됨
200 Reactions
카탈로그 번호 AB4106C
AB-4106/C으로도 사용됨
제품 가격(KRW)
1,477,000
온라인 행사
Ends: 30-Jun-2026
1,641,000
할인액 164,000 (10%)
Each
반응 수:
384 Reactions
제품 가격(KRW)
1,477,000
온라인 행사
Ends: 30-Jun-2026
1,641,000
할인액 164,000 (10%)
Each
The Thermo Scientific Verso 1-step RT-qPCR Low ROX Kit for SYBR Green chemistry offers a quick and simple way to detect mRNA. The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures.

Features of the Verso 1-step RT-qPCR SYBR Green Low ROX Kit:

• Eliminate the need for DNase treatments—RT Enhancer (optional) prevents gDNA carry-over
• RT and qPCR steps performed in single tube—convenience and reduced contamination risk
• Use of the entire RT reaction as template for qPCR—improved sensitivity
• Verso RT enzyme is highly sensitive with broad dynamic range—accurate detection of RNA from 1 pg-1 ug
• Verso RT enzyme has a wide working temperature range (42 to 57°C)—improved success with GC-rich and other difficult templates
• Contains an inert blue dye for ease of pipetting during reaction setup

An optional RT Enhancer is also provided and can be used during the RT step to remove any DNA contamination, eliminating the need for DNase I treatment. All Verso 1-step RT-qPCR kits incorporate an inert dye to significantly enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof.

In a 1-step approach to RT-qPCR, both the RT step and qPCR steps are performed in a single tube (1-step RT-qPCR). Adopting a 1-step approach is ideal for primer sequence optimization and collecting comparative data in experiments that do not require subsequent cDNA storage. The approach minimizes the risk of contamination, and improvements in reproducibility may be observed as a result of reduced sample handling.

Verso 1-Step RT-qPCR Kits for SYBR Green chemistry are compatible with all major real-time thermal cyclers.

Applications

• Gene expression analysis
• RNAi validation
• Microarray validation
• Pathogen detection
• Genetic testing
• Disease research

Related Products
Verso 1-step RT-qPCR Kit, SYBR Green, low ROX
Verso 1-step RT-qPCR Kit, SYBR Green, ROX
Verso 1-step RT-qPCR Kit, SYBR Green, with separate ROX vial
For Research Use Only. Not for use in diagnostic procedures.
사양
용도(장비)7500 System, QuantStudio™, ViiA™ 7 System, Stratagene Mx4000, Stratagene Mx3000P, Stratagene Mx3005P
형식Tube
반응 수384 Reactions
제품라인Verso
제품 유형Real Time PCR Kit
수량400 reactions
샘플 종류RNA
부피100 μL
검출 방법SYBR
GC-Rich PCR PerformanceHigh
PCR 방법1-step RT-qPCR
Unit SizeEach
구성 및 보관
Contains:
• 1 x 100 μL Verso Enzyme Mix
• 1 x 500 μL RT enhancer
• 1 x 5 mL 2X 1-Step qPCR SYBR Low ROX Mix

Store at -20°C

자주 묻는 질문(FAQ)

What is the RT Enhancer that is included in all Verso kits?

The RT Enhancer is a unique enzyme that degrades contaminating genomic DNA at the start of the RT step. It is equally as effective as DNase I treatment but more convenient as it eliminates the need for a separate DNase I incubation prior to RT-PCR.

Do I need to treat my RNA with DNase I before running RT-qPCR with Verso cDNA Synthesis Kit?

No, you do not need to treat your RNA with DNase I before running RT-qPCR with the Verso cDNA Synthesis Kit. The RT enhancer included in the Verso kit degrades any contaminating double-stranded DNA during the reverse transcription process, eliminating the need for a separate DNase I treatment.

Can I purchase the Verso Enzyme Mix separately from the Verso 1-step RT-qPCR Kit (Cat. No. AB4016A, AB4016C)?

No, we do not offer the Verso Enzyme Mix as a standalone product. It is only available as a component of the Verso 1-step RT-qPCR Kit (Cat. No. AB4016A, AB4016C).