Thermo Scientific™

ABsolute Blue QPCR Mix, with separate ROX vial

Catalog number:  AB4136B

 Related applications:

Real Time PCR (qPCR)

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Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of ABsolute Blue QPCR Mix and added additional capabilities for your gene expression analysis.

Thermo Scientific ABsolute Blue qPCR Mixes are 2X master mixes for probe chemistry. There are suitable alternatives available for all common real-time instruments. ABsolute Blue qPCR Mixes utilize Thermo-Start Taq hot start enzyme for increased sensitivity. The master mixes incorporate an inert blue dye to enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof.


High performance—ABsolute sensitivity, consistency & convenience
Enhanced contrast and reduced errors—easily identify correctly aliquotted reaction mix in all plates and tubes
Universal—compatible with all major qPCR platforms
Adaptable—available with different ROX passive reference dye concentrations


• Gene expression analysis
• siRNA validation
• Genotyping

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For Research Use Only. Not for use in diagnostic procedures.


Detection Method: Primer-Probe Detection
For Use With (Equipment): Bio-Rad CFX384 Touch™, BioRad CFX96™, BioRad MyiQ, BioRad iCycler iQ, BioRad iQ5, Cepheid SmartCycler, Corbett RotorGene, Eppendorf MasterCycler RealPlex, MJ Chromo4, MJ Opticon, Roche LightCycler 480
Reaction Speed: Standard
PCR Method: qPCR
Sample Type (General): DNA, dsDNA
Number of Reactions: 1,600 Reactions
Polymerase: Taq DNA Polymerase
Product Line: ABsolute Blue
Product Size: 1,600 reactions

Contents & storage

• 16 x 1.25 mL ABsolute Blue QPCR Mix (2X)
• 100 µL MgCl2 (1M)
• 25 µL ROX Reference Dye (1 mM)

Store at -20°C


Manuals & protocols