MagMAX™-96 Viral RNA Isolation Kit

Catalog number: AM1836

Applied Biosystems™  Related applications: Animal Health | Viral RNA⁄DNA Purification

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Description

Ambion's MagMAX™ Viral RNA isolation kits have been optimized for use with biological fluids and cell-free samples such as serum, plasma, swabs, and cell culture media. RNA isolation from cell-free samples presents some unique challenges compared to RNA isolation from tissues or cultured cells. These include low viral titers, large volumes to be processed, and sample complexity. Also, the procedure must be compatible with real-time qRT-PCR. The MagMAX™ Viral RNA isolation kits address all of these issues, without the need for organic solvents or RNA precipitation, eliminating the problems often encountered with filter-based RNA isolation methods such as clogging, large elution volumes, and inconsistent yields.

• More effective recovery of viral RNA and DNA from diverse sample types
• Improved qRT-PCR results
• Ideal when working with low viral concentrations
• Improved yields and reproducibility


MagMAX™-96 Viral RNA Isolation Kit
This high-throughput (96 well plate format) version of the improved MagMAX™ Viral RNA Isolation Kit also provides better viral particle lysis and more effective recovery of both viral RNA and DNA from a variety of cell-free biological samples. With this kit, DNA and RNA input levels ranging from 20 copies to 25 x 106 copies have been quantitatively recovered from plasma, serum, and milk. And even with less sample input, pathogen detection was as sensitive (equivalent Cts) or more sensitive (lower Cts) using nucleic acid isolated with the MagMAX™ kit when compared to nucleic acid isolated using another company's product.

For a NVSL-validated version of this kit, please see MagMAX™-96 AI⁄ND Viral RNA Isolation Kit.

Accessory Products
Lysis⁄Binding Solution Concentrate, Wash Solution 1 Concentrate, Wash Solution 2 Concentrate, and Carrier RNA are components of the MagMAX™ Viral RNA Isolation Kits that may also be purchased separately.

Additional Processing Plates and Lids: we recommend 96-well plates and lids from Phenix Research Products (#MPU-8117, #MPE-8019).

Benefits of Magnetic Bead-based Isolation
Magnetic beads offer many benefits for isolating RNA from cells and viral RNA from cell-free samples. Ambion’s MagMAX™technology binds RNA more efficiently than glass fiber filter methods resulting in higher RNA yields. The RNA yields are more consistent, both from experiment to experiment, and over a broad range of sample sizes. MagMAX™ magnetic bead technology eliminates filter clogging due to cellular particulates in samples. Since only a small volume of magnetic beads is needed for high efficiency binding, the bound RNA can be eluted in just 20–50 µl of nuclease free water. This makes theMagMAX™ magnetic bead technology extremely useful for concentrating RNA from large, dilute samples.
For Veterinary Use Only.

Specifications

Sample Type (General): Liquid Samples (e.g. Serum), Viral Samples
Sample Type (Specific): Cell-Free Fluids
Final Product: Viral DNA⁄RNA
Downstream Application: Reverse Transcriptase PCR (RT-PCR), Real-Time Quantitative PCR (qPCR), Northern Blotting
For Use With (Equipment): MagMAX™ Express-96 Standard Magnetic Particle Processor
High Throughput Compatibility: Automated Protocols, Not High Throughput-Compatible (Manual), High Throughput-Compatible
Isolation Technology: Magnetic Bead
Scale: Midi
Format: 96-well plate
Product Size: 96 preps
Yield (up to): 5 ng
Purification Time: 45 min
Number of Reactions: 96 Reactions
Starting Material (Amount): Up to 50µl of sample

Contents & storage

Carrier RNA is stored at –20°C; RNA Binding Beads are stored at 4°C. Wash Solution 2 Concentrate and Elution Buffer may be stored at either 4°C or room temperature. Lysis⁄Binding Solution Concentrate, Wash Solution 1 Concentrate, RNA Rebinding Concentrate, Bead ResuspensionSolution, Processing Plates and Lids, and PBS Buffer are stored at room temperature. Nuclease-free water may be stored at any temperature.

Documents

Manuals & protocols

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