EVOS™ Light Cube, Cy™7 - FAQs

EVOS light cubes, which enable fluorescent detection in the Countess 3 FL Automated Cell Counter, are compatible with a broad range of fluorescent dyes. Refer to our selection guide for an extensive list of compatible dyes. We also offer the ready-to-use ReadyCount Stains for easy viability staining.

Find additional tips, troubleshooting help, and resources within our Cell Counting Support Center.

Countess 3 Automated Cell Counters produce accurate and precise counts across a range of cell types, including human and murine peripheral blood mononuclear cells (PBMCs). Cell types and isolated nuclei smaller than 5 microns in diameter may be counted in FL only mode. Fluorescent staining, compatible EVOS light cubes, and update to the latest software version is required.

For more information on EVOS Light Cubes and compatible dyes, please use the following link:

EVOS Cell Imaging System Accessories

Find additional tips, troubleshooting help, and resources within our Cell Counting Support Center.

The GFP light cube (Ex = 470/22; Em = 510/42) has excitation centered at 470 nm covering 11 nm on each side for a total excitation range from 459 to 481 nm. Its emission is centered at 510 nm with 21 nm on each side for a total emission bandpass range from 489 to 531 nm.
The Qdot 525 light cube (Ex = 445/45; Em = 525/15) has excitation at 445 nm with 22.5 nm on each side for a total excitation range from 422.5 to 467.5. Its emission is centered at 525 nm with 7.5 nm on each side for a total emission bandpass range from 517.5 to 532.5.
The GFP light cube is best suited for fluorophores that are efficiently excited from 460 to 480 nm and emission with in the blue-green to green range.
The Qdot 525 light cube is better suited for exciting quantum dots at lower wavelengths (higher energy) and provides a narrower emission in the green range.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.