The EVOS M7000 Imaging System, High-Content Analysis Package, combines the fully automated, inverted, multi-channel fluorescence and transmitted light imaging system with Celleste 6.0 Image Analysis Software, a full-featured image analysis suite with protocols optimized to deliver the most relevant data for a range of popular live and fixed cell-based assays. The EVOS M7000 system was developed to meet today’s expectations for publication-quality images and data with just a few clicks using its powerful and time-saving acquisition tools with enhanced autofocus algorithms and automated routines for microwell plate assays, time-lapse live cell imaging, area scanning in time lapse mode, and Z-stacks in scan mode.
Celleste 6.0 software allows you to customize image analysis to your specific needs or take advantage of preconfigured analysis templates optimally designed to segment and classify images from a range of common cell-based assays. The union of the Celleste 6.0 software and EVOS microscope empowers you to easily transition from performing traditional microscopy to high-content analyses.
The EVOS M7000 High-Content Analysis packages offer:
• Highly configurable microscope—with a five-position objective turret and the ability to simultaneously acquire images in four fluorescence channels plus transmitted light with magnification from 1.25X to 100X, the optical system can be easily configured to meet your sample needs
• Publication-ready imagery in a few clicks—including autofocus, stitching, tiling, Z-stack capability, time-lapse imaging and single-click multichannel capture
• Intuitive acquisition interface—easily program the EVOS M7000 system to run well-plate scanning, time lapse experiments, and tile-stitch/montage area scans in Z-stack and/or time-lapse modes in your vessel of choice
• Streamlined image analysis—Celleste 6.0 icon-based wizard-drive workflow eliminates guesswork when using the preconfigured and optimized analysis templates for common applications to help derive the most relevant results from image sets
Fully automated imaging system
The EVOS M7000 system combines and integrates precision components with a unique modern design functionality that enables high-quality automated fluorescence imaging anywhere with workflow efficiency. Full automation of the precision X/Y-stage movement; changing of the four LED fluorescent light cubes, five-position objective turret, focus, and exposure; and switching of the dual camera make the EVOS M7000 system an exceptional platform for a variety of demanding imaging applications.
Through the intuitive acquisition interface, you can program the EVOS M7000 system to run well-plate scanning, time lapse experiments, and tile-stitch/montage area scans in Z-stack and/or time-lapse modes in your vessel of choice. For live cell imaging over multiple hours or days, the optional EVOS Onstage Incubator, which functions as an environmental chamber on top of the microscope stage, is operated seamlessly from within the instrument interface.
Versatile and highly configurable
Choose from our broad range of high quality coverslip-corrected and long-working-distance objectives for use with plastic vessels or glass sides. Available from 1.25X to 100X magnification in achromat, fluorite, or apochromat models, together with over twenty LED light cubes, the sample imaging options are limitless.
Dual monochrome and color high-sensitivity dual CMOS cameras enable seamless imaging of both fluorescent and chromogenically stained samples.
Interchangeable vessel holders accommodate most vessel types and sizes, including slides, multi-well plates, culture flasks, and Petri dishes, and afford precise control and sample alignment by the automated stage. The vessel creation wizard in the user interface enables creation of profiles for custom sample vessels.
Smart LED illumination technology
All EVOS fluorescence imaging systems utilize our proprietary LED light cubes. This light engine outputs remarkable intensity over a short light path and delivers fluorophore excitation. Each light cube contains a precisely matched set of optical components to optimize the position, evenness, and intensity of the light beam. Digitally controlled LED light sources allow adjustment of illumination levels to the sample and experimental conditions to minimize the risk of photo-toxicity and photo-bleaching in time-lapse studies. Hard-coated filters give sharper edges and significantly higher transmission efficiencies than traditional soft-coated filters.
Celleste Image analysis
The Celleste 6 Multi Channel Analysis (MCA) tool is based on pre-configured algorithms and analysis templates that have been trained on representative data to optimally segment and classify images from a range of common cell-based assays. Simply choose the app that corresponds to your assay of interest and follow the wizard-based workflow step by step from image to data generation.
Inspect images across the plate by clicking them or running the 'play' function. Then simply apply the analysis to the desired wells and immediately view results in a format most appropriate for the assay. Choose from block diagram, graph, heat map, histogram, pie chart, scatter plot, or 3D. Refine as desired and apply to the desired fields and wells in the data set.
Measure and quantify
Easily measure and analyze your images with a variety of measurement tools such as distance, region, angles, and area. The ability to identify an object in time-lapse experiments can be used to track cell movement or migration. It can also be used to track intensity changes over time, such as in cell death or target gene expression studies. This feature is particularly powerful in combination with the EVOS Onstage Incubator that allows continuous monitoring of cells under controlled environmental conditions.
Report and share
Upon completing the image analysis, a suite of annotation and reporting tools allow you to create presentation-ready images and data reports with a few clicks and to share them with others in PDF, PowerPoint™, and Excel™ format. Save images as individual frames or movies according to your needs.
Celleste 6 software is a powerful image analysis solution that helps make your imaging research job easier and increases your productivity by seamlessly providing images and accurate data. With the proven usability of EVOS imaging systems, you now have the tools that make it easy to capture, process, measure, analyze, and share your important images and data more effectively than ever.
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System and hardware specifications
Optics: infinity‐corrected optical system; RMS‐threaded objectives with 45-mm parfocal distance
Imaging mode: fluorescence, brightfield, color brightfield, and phase contrast
Illumination: five-position chamber for four fluorescence light cubes plus brightfield; light cubes with integrated hard coated filter set and LED light source with >50,000 hour life; broad selection of standard and specialty light cubes
Imaging methods: single-color, multi-color, area scan with montage or tile-stitch, time lapse, Z-stacking, movie capture
Objective capacity: five‐position turret
Objectives (not included): wide selection of high‐quality LWD and coverslip‐corrected objectives
Condenser: 60-mm long working distance condenser, four-position turret with a clear aperture and three phase annuli
Stage: motorized X/Y scanning stage; travel range 120 mm x 80 mm with sub‐micron resolution, drop‐in inserts to receive vessel holders and lock-down holders to fix sample in place during long scans
Focus mechanism: automated focus mechanism with sub‐micron resolution
LCD display: 23-inch high‐resolution touch screen color monitor (also fully controllable via mouse); 1920 x 1080 pixel resolution
Cameras: high-sensitivity 3.2 MP (2048 x 1536) monochrome CMOS sensor with 3.45 µm pixel resolution