Invitrogen™

Click-IT™ L-Homopropargylglycine (HPG) This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number:  C10186

Related applications:

Labeling Chemistry

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Description

Click-iT® homopropargylglycine (HPG) provides a fast, sensitive, non-toxic and most importantly non-radioactive alternative to the traditional technique,35S methionine for the detection of nascent protein synthesis. HPG is an amino acid analog that contains a very small modification, specifically an alkyne moiety that can be fed to cultured cells and incorporated into proteins during active protein synthesis. Detection utilizes the chemoselective ligation or “click" reaction between and azide and an alkyne where the alkyne modified protein is detected with an azide-containing dye or hapten together with either the Click-iT® Cell Reaction Buffer Kit or the Click-iT® Protein Buffer Kit. With the Click-iT® Cell Reaction Buffer Kit, cells can be analyzed by fluorescence microscopy, flow cytometry or high-content imaging and analysis. With the Click-iT® Protein Reaction Buffer Kit, achieve detection sensitivity in 1-D gels and western blots in the low femtomole range or perform LC-MS⁄MS and MALDI MS analysis.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Form: Solid
Labeling Method: Metabolic Labeling
Labeling Target: Proteins (Nascent)
Shipping Condition: Room Temperature
Product Line: Click-iT™, Molecular Probes®
Label or Dye: Alkyne
Green Features: Less hazardous

Contents & storage

Store at ≤-20°C and desiccated.