Now Available in Complete Kits: Introducing the Click-iT HPG Alexa Fluor® 488 and Alexa Fluor® 594 kits
Use Prolong Diamond with any Fluorescent Dye or Fluorescent Protein for brightest images. Learn more.

Click-IT™ L-Homopropargylglycine (HPG) This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number:  C10186

 Related applications:

Labeling Chemistry

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}} also known as {{product.formattedSku}} 
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top


Click-iT® homopropargylglycine (HPG) provides a fast, sensitive, non-toxic and most importantly non-radioactive alternative to the traditional technique,35S methionine for the detection of nascent protein synthesis. HPG is an amino acid analog that contains a very small modification, specifically an alkyne moiety that can be fed to cultured cells and incorporated into proteins during active protein synthesis. Detection utilizes the chemoselective ligation or “click" reaction between and azide and an alkyne where the alkyne modified protein is detected with an azide-containing dye or hapten together with either the Click-iT® Cell Reaction Buffer Kit or the Click-iT® Protein Buffer Kit. With the Click-iT® Cell Reaction Buffer Kit, cells can be analyzed by fluorescence microscopy, flow cytometry or high-content imaging and analysis. With the Click-iT® Protein Reaction Buffer Kit, achieve detection sensitivity in 1-D gels and western blots in the low femtomole range or perform LC-MS⁄MS and MALDI MS analysis.
For Research Use Only. Not for use in diagnostic procedures.


Form: Solid
Green Features: Less hazardous
Label or Dye: Alkyne
Labeling Method: Metabolic Labeling
Labeling Target: Proteins (Nascent)
Product Line: Click-iT™, Molecular Probes®
Product Size: 5 mg
Shipping Condition: Room Temperature

Contents & storage

Store at ≤-20°C and desiccated.


Manuals & protocols