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Click-IT® Biotin Protein Analysis Detection Kit This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number: C33372

Molecular Probes™  Related applications: Labeling Chemistry | Protein Assays and Analysis | Protein Gel Electrophoresis

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The Click-iT® Biotin Glycoprotein Detection Kit provides the second part of a simple and robust technique to identify and characterize glycoproteins by Western blot. In step two, after the incorporation of an azide moiety into protein glycan structures with either a Click-iT® metabolic labeling reagent or the Click-iT® Enzymatic Labeling system, the azide-modified glycoproteins are detected via the chemoselective ligation or click reaction between an azide and an alkyne. With this technique, detection sensitivity is in the low femptomole range and biotin-labeled samples can be detected before or after probing the Western with a primary antibody.
For Research Use Only. Not for use in diagnostic procedures.


Label or Dye: Biotin
Product Size: 1 kit
Labeling Target: Proteins (Glycoproteins)
Detection Method: Fluorescent, Biotin-Based
Green Features: Less hazardous
Shipping Condition: Room Temperature

Contents & storage

Store in freezer (-5 to -30°C).


Manuals & protocols

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