The fluorogenic DQ™ collagen can be used to directly monitor collagenase activity. DQ™ substrates are analogs of the natural substrate that have an excessive number of fluorescent dyes attached so that the fluorescence signal is almost non-existent. This quenching of the signal is caused by the close proximity of the dyes on the intact substrate. The enzyme-driven hydrolysis of the substrate results in separation of the dye results in separation of the dye molecules from one another and the fluorescence signal increases.

See User Manual for solubility instructions.