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Citas y referencias (37)
Invitrogen™
Fluorescein Diphosphate, Tetraammonium Salt (FDP)
FDP es un sustrato incoloro y no fluorescente para fosfatasas alcalinas. La hidrólisis secuencial mediada por fosfatasa alcalina de susMás información
| Número de catálogo | Cantidad |
|---|---|
| F2999 | 5 mg |
Número de catálogo F2999
Precio (MXN)
-
Cantidad:
5 mg
FDP es un sustrato incoloro y no fluorescente para fosfatasas alcalinas. La hidrólisis secuencial mediada por fosfatasa alcalina de sus dos sustituyentes de fosfato produce un monofosfato de fluoresceína poco fluorescente seguido de fluoresceína muy fluorescente (excitación/emisión ∼ 490/514 nm).
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Excitación/emisión490⁄514
Para utilizar con (equipo)Fluorímetro, lector de microplacas
Etiqueta o tinteFITC (fluoresceína)
Cantidad5 mg
Condiciones de envíoTemperatura ambiente
SustratoSustrato de fosfatasa
Método de detecciónFluorescente
FormularioPolvo
Substrate PropertiesSustrato químico
Target EnzymeFosfatasa alcalina, fosfatasas de tirosina
Unit SizeEach
Contenido y almacenamiento
Almacenar en congelador (de - 5 a - 30 °C).
Citations & References (37)
Citations & References
Abstract
Glibenclamide prevents increased extracellular matrix formation induced by high glucose concentration in mesangial cells.
Journal:Am J Physiol Renal Physiol
PubMed ID:16896180
'Other than stimulation of cell contractility, little is known about the potential metabolic effects induced by sulfonylureas, independently of insulin action. Previous studies from our laboratory demonstrated complete abrogation of glomerulosclerosis in an experimental model of type 1 diabetes chronically (9 mo) treated with low-dose sulfonylureas (Biederman JI, Vera E,
Negative regulation of EGFR signalling through integrin-alpha1beta1-mediated activation of protein tyrosine phosphatase TCPTP.
Journal:Nat Cell Biol
PubMed ID:15592458
'Integrin-mediated cell adhesion regulates a multitude of cellular responses, including proliferation, survival and cross-talk between different cellular signalling pathways. So far, integrins have been mainly shown to convey permissive signals enabling anchorage-dependent receptor tyrosine kinase signalling. Here we show that a collagen-binding integrin alpha(1)beta(1) functions as a negative regulator of
Functional screening of intracellular proteins in single cells and in patterned cell arrays using electroporation.
Journal:Anal Chem
PubMed ID:12199607
'A tool for detection and characterization of intracellular enzyme-substrate and receptor-ligand interactions inside the cytoplasm of single targeted cells or small confined groups of cells is presented. Fluorogenic enzyme substrates and receptor ligands were rapidly delivered by electroosmosis and internalized by electroporation in cells using an electrolyte-filled capillary (EFC) biased
Randomly ordered addressable high-density optical sensor arrays.
Journal:Anal Chem
PubMed ID:9553489
'Array-based sensors provide an architecture for multianalyte sensing. In this paper, we report a new approach for array fabrication. Sensors are made by immobilizing different reactive chemistries on the surfaces of microspheres. Sensor arrays are prepared by randomly distributing a mixture of microsphere sensors on an optical substrate containing thousands
In vivo imaging of tumors with protease-activated near-infrared fluorescent probes.
Journal:Nat Biotechnol
PubMed ID:10207887
'We have developed a method to image tumor-associated lysosomal protease activity in a xenograft mouse model in vivo using autoquenched near-infrared fluorescence (NIRF) probes. NIRF probes were bound to a long circulating graft copolymer consisting of poly-L-lysine and methoxypolyethylene glycol succinate. Following intravenous injection, the NIRF probe carrier accumulated in