DyNAmo ColorFlash SYBR Green qPCR Kit
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Thermo Scientific™

DyNAmo ColorFlash SYBR Green qPCR Kit

**********************Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at aRead more
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Catalog NumberNo. of Reactions
F416L
also known as F-416L
500 reactions
F416XL2500 Reactions
Catalog number F416L
also known as F-416L
Price (USD)
620.00
Each
Add to cart
No. of Reactions:
500 reactions
Price (USD)
620.00
Each
Add to cart
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Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of DyNAmo ColorFlash SYBR Green qPCR Kit and added additional capabilities for your gene expression analysis.

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Thermo Scientific DyNAmo ColorFlash SYBR Green qPCR Kit offers equal performance to the DyNAmo Flash SYBR Green qPCR Kit. In addition, it incorporates an innovative multi-color system that ensures correct pipetting. The 2X master mix contains a blue dye, and a separate sample buffer contains a yellow dye. The qPCR reaction mix containing both components is green. Using this patent-pending multicolor system, pipetting of both the master mix and the sample can be easily monitored. This significantly decreases the risk for pipetting errors during reaction setup, especially when using white reaction vessels. The dyes do not affect the specificity or sensitivity of qPCR assays.

Highlights

• Colored reagents minimize the risk of pipetting errors during reaction set up
• Especially beneficial when using white reaction vessels
• Extremely fast protocols due to combined annealing and extension step of only 15 s
• Specific and sensitive detection of a wide range of template concentrations
• dUTP included in the 2x master mix allows the use of UNG for prevention of carry-over contamination

Applications

• Fast qPCR
• qPCR using SYBR Green dye
• RT-qPCR using SYBR Green dye

Related Products
DyNamo ColorFlash Probe qPCR Kit
DyNAmo ColorFlash SYBR Green qPCR Kit
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Equipment)7000 System, 7300 System, 7500 Fast System, 7500 System, 7700 System, 7900HT Fast System, 7900HT System, BioRad CFX96™, QuantStudio™, ViiA™ 7 System, Bio-Rad CFX384 Touch™, Roche LightCycler 480, 5700 Sequence Detection System, StepOnePlus™ System, Eppendorf MasterCycler RealPlex, Corbett RotorGene, StepOne™ System, Stratagene Mx4000, Stratagene Mx3005P, Stratagene Mx3000P, MJ Opticon, MJ Chromo4, Cepheid SmartCycler
FormatTube
Kit TypeDyNAmo ColorFlash SYBR Green qPCR Kit
No. of Reactions500 reactions
PolymeraseTbr DNA polymerase
Product LineDyNAmo Flash
Product TypeReal Time PCR SYBR Master Mix
Quantity500 reactions
Sample TypeDNA, dsDNA
Sufficient For500 Reactions of 20 μL, 200 Reactions of 50 μL
Volume5 x 1 mL
Concentration2X (qPCR Mix), 50X (Dye), 40X (Buffer)
Detection MethodSYBR
For Use With (Application)Gene Expression
GC-Rich PCR PerformanceHigh
PCR MethodqPCR
Reaction SpeedFast
Unit SizeEach
Contents & Storage
Contains:
• 5 x1 mL DyNAmo ColorFlash SYBR Green qPCR Mix (2X)
• 1 x 250 μL 50 x ROX dye, 25μM
• 1 x 1000 μL 40x sample buffer solution with yellow dye

Store at -20°C

Frequently asked questions (FAQs)

Does the yellow dye from the Yellow Sample Buffer provided with Luminaris Color and DyNAmo Flash qPCR Master Mixes influence qPCR performance?

The addition of yellow dye from the Yellow Sample Buffer to qPCR reaction has no influence on the efficiency, specificity, sensitivity or Cq values. Using the Yellow Sample Buffer is optional, but the dye helps to see in which wells the sample has been already added.

Do I need to use Fast plastics to run a Fast qPCR reaction?

Specialized Fast PCR plates are not required if you are using a standard qPCR machine with a fast protocol. However, Fast Block PCR Plates (AB-1900) can be purchased which are compatible with the thermal blocks on fast qPCR thermal cyclers.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.