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ImaGene Green™ C12FDG lacZ Gene Expression Kit

Catalog number: I2904

Molecular Probes™  Related applications: Cellular Imaging | Flow Cytometry | Protein Assays and Analysis

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Description

The ImaGene Green™ lacZ Gene Expression Kit contains a fluorescein-based galactosidase substrate that has been covalently modified to include a 12-carbon lipophilic moiety, C12FDG. Once inside the cell, the substrates are cleaved by β-galactosidase, producing a fluorescent product that is well retained by the cells, probably by incorporation of the lipophilic tail within the cellular membrane. In addition to the C12FDG, the kits also include the broad-spectrum β-galactosidase inhibitor, PETG, and chloroquine diphosphate for inhibiting acidic hydrolysis of the substrate.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay Type: β-Gal (lacZ) Assay
Detection Method: Fluorescent
Excitation⁄Emission (nm): 497⁄518
Product Size: 1 kit
Substrate: C12FDG
Substrate Properties: Chemical Substrate
Substrate Type: Beta-Gal Substrate
Target Enzyme: Beta-Galactosidase
Shipping Condition: Room Temperature

Contents & storage

Store in freezer (-5 to -30°C) and protect from light.

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