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This product is animal origin and can only be shipped within the United States
Elastase, porcine pancreas
Thermo Scientific Chemicals

Elastase, porcine pancreas

Elastase, porcine pancreas, CAS # 39445-21-1, is a single polypeptide chain of 240 amino acid residues isolated from the porcine pancreas. It is a serine protease that degrades elastin, a matrix component of tissues. | CAS: 39445-21-125 kd g/mol
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Catalog number J61874.03
also known as J61874-03
Price (USD)
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Quantity:
1 g
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Chemical Identifiers
CAS39445-21-1
SpecificationsSpecification SheetSpecification Sheet
Appearance (Color)White to pale cream
FormLyophilized powder
Unit definitionOne unit cleaves one micromole of N-succinyl-L-alanyl-L-alanyl-L-alanine-p-nitroanalide per minute at 25°C, pH 8.0
units/mgdw>=3 units
UV/VisAA280 (mg/ml): Report value
This enzyme can be used alone or in combination with other proteases for protein analysis by mass spectrometry and other applications. Elastase, is used in tissue dissociation because elastin is found in highest concentrations in the elastic fibers of connective tissues, elastase is frequently used to dissociate tissues that contain extensive intercellular fiber networks. For this purpose, it is usually used with other enzymes such as collagenase, trypsin, and chymotrypsin, membrane protein solubilization, protein sequence studies.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General Description

• Elastase is a serine protease that can hydrolyze elastin, a specific protein of elastic fibers

Application

• Elastase, porcine pancreas has been used in cell culture techniques, ELISA assays, and in experiments to measure the inhibitory activity of elastase

• Since elastin is found in the fibers of connective tissues, elastase is commonly used to dissociate tissues that contain extensive intercellular fiber networks

• It is typically used with other enzymes, including collagenase, trypsin, and chymotrypsin

• Elastase enzyme is isolated from porcine pancreas and can be used alone or combined with other proteases for protein analysis by mass spectrometry and other applications, including membrane protein solubilization and protein sequence studies

• This compound also hydrolyzes amides and esters such as N-Benzoyl-L-alanine methyl ester

RUO – Research Use Only

General References:

  1. RD Hautamaki.; DK Kobayashi.; RM Senior.; SD Shapiro. Requirement for macrophage elastase for cigarette smoke-induced emphysema in mice. Science. 1997, 277, (5334), 2002-2004.
  2. J Bieth.; B Spiess.; CG Wermuth. The synthesis and analytical use of a highly sensitive and convenient substrate of elastase. Biochemical medicine. 1974, 11, (4), 350-357.
  3. Azmi N, Hashim P, Hashim DM, Halimoon N, Majid NM. Anti-elastase, anti-tyrosinase and matrix metalloproteinase-1 inhibitory activity of earthworm extracts as potential new anti-aging agent. Asian Pac J Trop Biomed. 2014, 4 (Suppl 1), S348-352.
  4. Kraunsoe JA, Claridge TD, Lowe G. Inhibition of human leukocyte and porcine pancreatic elastase by homologues of bovine pancreatic trypsin inhibitor. Biochemistry. 1996, 35, (28), 9090-9096.
Contents & Storage
2°C to 8°C