Thermo Scientific™

RevertAid H Minus First Strand cDNA Synthesis Kit

Catalog number:  K1632

Related applications:

cDNA Libraries & Library Construction

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Thermo Scientific RevertAid H Minus First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit includes RevertAid H Minus Reverse Transcriptase, which has a point mutation that completely eliminates RNase H activity. Therefore, it does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA.


• High yields of full-length first strand cDNA up to 13 kb
• Increased reaction temperatures in the range of 42 to 55°C
• Supplied with the recombinant RiboLock RNase Inhibitor
• Complete kit—oligo(dT)18 and random hexamer primers included with the kit


• First strand cDNA synthesis for RT-PCR and real-time RT-PCR
• Construction of full length cDNA libraries
• Antisense RNA synthesis

Additional Features

The recombinant RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA template from degradation. It is fully compatible with the reverse transcription reaction, as it maintains activity at temperatures up to 55°C.The kit is supplied with both oligo(dT)18 and random hexamer primers. The oligo(dT)18 primer anneals selectively to the poly(A) tail of mRNA. Random hexamer primers do not require the presence of poly(A), therefore, they can be used for transcription of the 5’-end regions of mRNA or for cDNA synthesis using RNA without the poly(A) tail, e.g., microRNAs. Gene-specific primers may also be used with the kit. The first strand cDNA can be directly used as a template in PCR, realtime PCR or in second strand cDNA synthesis.
For Research Use Only. Not for use in diagnostic procedures.


Optimal Reaction Temperature: 42-45° C
Product Line: RevertAid
Reverse Transcriptase: RevertAid H Minus
Sample Type: RNA
For Use With (Application): Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR)
No. of Reactions: 100 Reactions (Polymerase)
PCR Method: 2-Step Quantitative RT-PCR (RT-qPCR)
Product Type Specs: First Strand CDNA Synthesis Kit
Reaction Format: Separate Components
Sufficient For: 100 reactions

Contents & storage

The RevertAid H Minus First Strand cDNA Synthesis Kit contains:
• RevertAid H Minus Reverse Transcriptase,
• RiboLock RNase Inhibitor,
• 5X Reaction Buffer,
• dNTP Mix,
• Oligo(dT)18 Primer,
• Random Hexamer Primer,
• Control RNA,
• Control Primers and
• Nuclease-free water.

Store at -20°C.