ViraPower™ II Lentiviral Gateway® Expression System

Catalog number: K36720

Invitrogen™  Related applications: Gateway Cloning | Protein Expression

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The ViraPower™ II Lentiviral Gateway® Expression System includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit. This kit combines Invitrogen’s ViraPower™ II Lentiviral and Gateway® technologies to facilitate easy recombination-based cloning and lentiviral-based expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.2⁄V5-DEST™ vector in the kit has the CMV promoter for driving constitutive expression of the target gene in mammalian cells. The ViraPower™ II Lentiviral system uses the PGK promoter instead of SV40 promoter in the lentiviral vector for driving long-term, persistent expression of the blasticidin stable selection marker.

• Generates replication-incompetent lentivirus for transducing dividing and non-dividing mammalian cells
• Easy recombination-based cloning using Gateway® technology
• Stable expression, long term expression

Key Features
• CMV promoter for high level, constitutive expression of the target gene
• Blasticidin selection marker for stable selection under control of PGK promoter for long-term, persistent expression

Kit includes
• pLenti6.2⁄V5-DEST™ Gateway® Vector Kit (Cat # V36820)
• ViraPower™ Bsd Lentiviral Support Kit (Cat # K497000)
• 293FT Cell Line (Cat # R70007)

For research use only. Not intended for any therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.


Promoter: CMV
Key Function: Viral Expression
Protein Tag or Fusion: V5 Epitope Tag
Delivery Method: Lentiviral
Cloning Method: Gateway®
Selection Agent (Eukaryotic): Blasticidin
Vector Type: pLenti
Product Size: 1 kit
Constitutive or Inducible System: Constitutive

Contents & storage

pLenti6.2⁄V5-DEST™ Gateway® Vector Kit
• 6 µg of pLenti6.2⁄V5-DEST™ vector (40 µL of vector at 150 ng⁄µL in TE Buffer, pH 8.0)
• 10 µg of pLenti6.2⁄V5-GW⁄lacZ™ plasmid (20 µL of plasmid at 0.5 µg⁄µL in TE Buffer, pH 8.0)
• One Shot® Stbl3™ Chemically Competent E. coli (Cat # C737303): 21 x 50-µL of Stbl3™ Cells, 6 mL of S.O.C. Medium and 50 µL of pUC19 control DNA (10 pg⁄µL).

ViraPower™ Bsd Lentiviral Support Kit (Cat # K497000)
• 195 µg of ViraPower™ Packaging Mix (Contains 195 µL of a mixture of pLP1, pLP2, and pLP⁄VSVG plasmids, at 1 µg⁄µL in TE Buffer, pH 8.0)
• 0.75 ml of Lipofectamine® 2000 (Cat # 11668027)
• 50 mg of Blasticidin powder (Cat # R21001)
• 293FT Cell Line (Cat # R70007): 1 vial containing 3 X 106 frozen cells in 1 mL of Freezing Medium.

• Vectors: -20°C
• ViraPower™ Packaging Mix: -20°C
• Blasticidin: -20°C
• Lipofectamine® 2000: 4°C (do not freeze)
• One Shot® Stbl3™ Chemically Competent E. coli: -80°C
• 293 FT Cells: Liquid Nitrogen


Manuals & protocols