Invitrogen™

pENTR™ 5'-TOPO™ TA Cloning™ Kit, with One Shot™ TOP10 Chemically Competent E. coli

Catalog number:  K59120

Related applications:

Gateway Cloning

| Protein Expression

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Description

The pENTR™ 5’-TOPO® TA Cloning® Kit combines Invitrogen’s TOPO® Cloning and MultiSite Gateway® Technologies to facilitate five-minute, one-step cloning of a Taq polymerase-amplified PCR product encoding a eukaryotic promoter of interest into a MultiSite Gateway® entry vector with ≥ 85% efficiency. Once cloned, your eukaryotic promoter may be transferred from the pENTR™ 5'-TOPO® vector to a suitable MultiSite Gateway® destination vector in a MultiSite Gateway® LR recombination reaction with other suitable entry clone(s) to generate an expression construct.

This kit can be used in combination with ViraPower™ HiPerform Promoterless Lentiviral Gateway® Vector Kit, or MultiSite Gateway® Three-Fragment Vector Construction Kit.

Advantages
• Choose the best promoter for your experiments and cell line by efficiently expressing your gene of interest from multiple promoter constructs
• When using in conjunction with ViraPower™ HiPerform™ Promoterless Lentiviral vector, you can efficiently transduce and express your gene of interest driven by the promoter of choice in both dividing and non-dividing mammalian cells


Key Features
• Rapid PCR cloning with TOPO® cloning technology for cloning the 5’ element
• attL4 & attR1 sites for compatibility with MultiSite Gateway® LR reaction for increased flexibility

Kit Includes
• pENTR™ 5´-TOPO® TA Cloning® Kit
• One Shot® TOP10 Chemically Competent E. coli (C4040-03)

Related SKUs
• ViraPower™ HiPerform™ Promoterless Lentiviral Gateway® Expression System (A111451)
• ViraPower™ HiPerform™ Promoterless Gateway® Vector Kit (A11146)
• MultiSite Gateway® Three-Fragment Vector Construction Kit (12537-023)

For research use only. Not intended for any therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cloning Method: TOPO®-TA
Product Line: One Shot™
Product Size: 20 reactions
Vector Type: TOPO-TA Cloning Vectors,pENTR

Contents & storage

The pENTR™ 5´-TOPO® TA Cloning® Kit with One Shot® TOP10 Chemically Competent E. coli contains two boxes.

The TOPO® Cloning box contains enough linearized and topoisomerase I-activated pENTR™ 5´-TOPO® vector for 20 cloning reactions, dNTPs, salt solution, control template and primers and primers for sequencing. Store at -20°C.

The One Shot® TOP10 box contains transformation reagents including 20 single-use 50 µl aliquots of One Shot® Competent Cells, S.O.C. medium, and pUC19 supercoiled plasmid control. Store at -80°C.