NuPAGE® Tris-Acetate SDS Buffer Kit (for Tris-Acetate Gels), Contains 1 ea. LA0041, NP0004, NP0005, NP0007

Catalog number: LA0050

Novex™  Related applications: Protein Gel Electrophoresis

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Description

NuPAGE® Novex 3-8% Tris-Acetate Midi-Gels provide excellent separation of large molecular weight proteins when used with NuPAGE® Tris-Acetate SDS Running Buffer. NuPAGE® Novex Tris-Acetate gels can also be run with Tris-Glycine Native Running Buffer to resolve native proteins more effectively than the Tris-Glycine gel system.

NuPAGE® Novex Tris-Acetate Midi-Gels (8 x 13 cm) can run up to 26 samples. The NuPAGE Midi-Gels run in the XCell4 Surelock™ Midi-Cell and, with a Midi Gel Adapter, can run in a Bio-Rad Criterion Cell.

Formulation: NuPAGE® Novex Tris-Acetate gels are made with high-purity, strictly quality-controlled reagents: Tris base, acetic acid, acrylamide, bis-acrylamide, TEMED, APS, and highly purified water. They do not contain SDS.

Recommended Buffers: Run NuPAGE® Novex Tris-Acetate gels with Novex® Tris-Acetate SDS Running Buffer. To ensure good sample reduction and band resolution, use NuPAGE® Sample Preparation Reagents with these gels. The use of NuPAGE® Transfer Buffer provides optimal conditions for transfer of proteins to nitrocellulose, PVDF, or nylon membranes for subsequent analysis. For native running conditions, the Tris-Glycine Native Running and Sample Buffers should be used with NuPAGE® Tris-Acetate gels.

12+2 wells
Maximum sample volume/well 45 µl sample + 15 µl standards
Maximum protein load Coomassie® Stain 0.7 µg/band
Maximum protein load Silver Stain 1.4 ng/band

20 wells
Maximum sample volume/well 25 µl
Maximum protein load Coomassie® Stain 0.5 µg/band
Maximum protein load Silver Stain 1 ng/band

26 wells
Maximum sample volume/well 15 µl
Maximum protein load Coomassie® Stain 0.35 µg/band
Maximum protein load Silver Stain 0.7 ng/band

Currently using: Recommended NuPAGE® Gel:
4% Tris-Glycine NuPAGE® 3-8% Tris-Acetate (+ TA Buffer)
6% Tris-Glycine NuPAGE® 3-8% Tris-Acetate (+ TA Buffer)
10% Tris-Glycine NuPAGE® 8% Bis-Tris (+ MOPS Buffer)
or NuPAGE® 10% Bis-Tris (+ MOPS Buffer)
12% Tris-Glycine NuPAGE® 10% Bis-Tris (+ MOPS Buffer)
4-12% Tris-Glycine NuPAGE® 3-8% Tris-Acetate (+ TA Buffer)
or NuPAGE® 4-12% Bis-Tris (+ MOPS Buffer)
4-20% Tris-Glycine NuPAGE® 4-12% Bis-Tris (+ MES Buffer)
8-16% Tris-Glycine NuPAGE® 4-12% Bis-Tris (+ MOPS Buffer)
10% Tricine NuPAGE® 8% Bis-Tris (+ MES Buffer)
or NuPAGE® 10% Bis-Tris (+ MES Buffer)
16% Tricine NuPAGE® 4-12% Bis-Tris (+ MES Buffer)
10-20% Tricine NuPAGE® 4-12% Bis-Tris (+ MES Buffer)

For more information on the benefits of NuPAGE neutral pH technology go to www.invitrogen.com/nupage
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Gel Type: SDS PAGE Gel
Buffer Type: Sample Loading Buffers
Gel Compatibility: NuPAGE® Tris-Acetate Gels
Product Size: 1 kit

Contents & storage

NuPAGE® Tris-Acetate SDS Buffer Kit (for Tris-Acetate Gels) contains:

• 20X NuPAGE® Tris-Acetate SDS Running Buffer (500 ml): Store at 4°C to 25°C

• 10X NuPAGE® Sample Reducing Agent (250 µl): Store at 2°C to 8°C

• NuPAGE® Antioxidant (15 ml): Store at 4°C

• 4X NuPAGE® LDS Sample Buffer (10 ml): Store at 4°C to 25°C

Documents

Manuals & protocols

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