MUP (4-Methylumbelliferyl Phosphate, Free Acid)

Citations & References (37)

Invitrogen™

MUP (4-Methylumbelliferyl Phosphate, Free Acid)

MUPは、アルカリホスファターゼ用の蛍光基質です。アルカリホスファターゼは、そのリン酸置換基の加水分解を触媒します。これにより、青色蛍光4-メチルウンベリフェリル（励起／発光波長：約386/448 nm）が生じます。詳細を見る

製品番号（カタログ番号）	数量
M6491	1 g

製品番号（カタログ番号） M6491

価格（JPY）

28,800

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MUPは、アルカリホスファターゼ用の蛍光基質です。アルカリホスファターゼは、そのリン酸置換基の加水分解を触媒します。これにより、青色蛍光4-メチルウンベリフェリル（励起／発光波長：約386/448 nm）が生じます。

研究用にのみ使用できます。診断用には使用いただけません。

励起／発光360⁄449

使用対象 (装置)蛍光光度計、マイクロプレートリーダー

標識または色素MUP（4-メチルウンベリフェリルリン酸）

数量1 g

出荷条件室温

基質ホスファターゼ基質

検出法蛍光

Substrate Properties化学基質

Target Enzymeアルカリホスファターゼ

Unit SizeEach

組成および保存条件

冷凍庫（-5～-30℃）に保存。

引用および参考文献 (37)

引用および参考文献

Abstract

Enzyme-linked immunosorbent assay for an octapeptide based on a genetically engineered fusion protein.

Authors:Witkowski A, Daunert S, Kindy MS, Bachas LG

Journal:Anal Chem

PubMed ID:8503503

Traditional chemical means of preparing enzyme-ligand conjugates for use in enzyme-linked immunosorbent assays (ELISAs) lead to the production of multisubstituted enzyme-ligand conjugates with a high degree of variability in the site of ligand attachment. A genetically engineered fusion protein was prepared in order to investigate the feasibility of controlled production ... More

Rapid characterisation and identification of mycobacteria using fluorogenic enzyme tests.

Authors:Hamid ME, Chun J, Magee JG, Minnikin DE, Goodfellow M

Journal:Zentralbl Bakteriol

PubMed ID:8061408

'Sixty representatives of selected Mycobacterium and Nocardia species were examined for their ability to cleave 79 fluorogenic synthetic enzyme substrates based on the fluorophores 7-amino-4-methylcoumarin and 4-methylumbelliferone. The resultant data were analysed using the simple matching coefficient and clustering achieved using the unweighted pair group method with arithmetic averages algorithm. ... More

Fluorogenic selective and differential medium for isolation of fecal streptococci.

Authors:Littel KJ, Hartman PA

Journal:Appl Environ Microbiol

PubMed ID:6830220

'Of 44 fluorogenic substrates tested for their ability to differentiate species of fecal streptococci, four yielded species-differentiating reactions. The remaining substrates either yielded uniformly positive, negative, or variable strain-dependent reactions. One substrate, 4-methylumbelliferone-alpha-D-galactoside, was hydrolyzed by Streptococcus bovis and S. faecium and its biotypes. 4-Methylumbelliferone-alpha-D-galactoside and a colorimetric starch substrate ... More

Enhanced sensitivity and precision in an enzyme-linked immunosorbent assay with fluorogenic substrates compared with commonly used chromogenic substrates.

Authors:Meng Y, High K, Antonello J, Washabaugh MW, Zhao Q

Journal:Anal Biochem

PubMed ID:16137635

'Quantitative enzyme-linked immunosorbent assay (ELISA) is a widely used tool for analyzing biopharmaceutical and vaccine products. The superior sensitivity of the ELISA format is conferred by signal amplification through the enzymatic oxidation or hydrolysis of substrates to products with enhanced color or fluorescence. The extinction coefficient for a colored product ... More

Sensitive fluorogenic enzyme immunoassay on nitrocellulose membranes for quantitation of virus.

Authors:Fulton RE, Wong JP, Siddiqui YM, Tso MS

Journal:J Virol Methods

PubMed ID:3146582

'A highly sensitive fluorogenic enzyme-linked immunosorbent assay (FELISA), which utilizes nitrocellulose membranes as solid phase support, has been developed for the detection and identification of virus in clinical samples. Reagents were standardized and, using purified Newcastle disease virus (NDV) as a model, the theoretical lower limits of test sensitivity of ... More

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