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Invitrogen
CD11b Monoclonal Antibody (CA16.3E10)
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FIGURE: 1 / 1
CD11b Antibody (MA5-16604) in Flow
Stem cells international 2020 -
Figure 3 Flow cytometry for the isolated cells. (a) Representative histograms demonstrating positive and negative staining of urine-derived cells from a single dog. (b) Percentage of positive cells for the urine-derived cells isolated from three canine donors.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-16604
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Cat,
Goat,
Mustelid,
Mink,
Pig
Published species
Bovine,
Dog
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
CA16.3E10
Immunogen
Affinity purified beta-2 integrins from splenic lysate
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
tissue culture supernatant
Contains
<0.1% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommend the use of acetone fixation for frozen sections. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
Mouse anti Dog CD11b antibody, clone CA16.3E10 is a monoclonal antibody recognising the canine CD11b cell surface antigen, a member of the alpha integrin family.
Target Information
CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions. CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: antigen CD11b (p170); macrophage antigen alpha polypeptide; integrin alpha M; integrin, alpha M (complement component 3 receptor 3 subunit); leukocyte integrin alpha-M chain; Mac-1 alpha (Mac1A); MGC117044
Gene Aliases: CD11B
Entrez Gene ID: (Pig) 397459, (Goat) 102180100, (Bovine) 407124, (Dog) 489928
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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