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Invitrogen
E-cadherin Recombinant Rabbit Monoclonal Antibody (117)
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FIGURE: 1 / 1
E-cadherin Antibody (MA5-29711) in IP
E-cadherin Immunoprecipitation using: Lane A: 0.5 mg MCF-7 Whole Cell Lysate, Lane B: 0.5 mg A431 Whole Cell Lysate 4 µL with E-cadherin Recombinant Rabbit Monoclonal Antibody (117) (Product # MA5-29711) and 15 µL of 50 % Protein G agarose. Primary antibody: E-cadherin Recombinant Rabbit Monoclonal Antibody (117), at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 130 kDa. Observed band... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-29711
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
117
Immunogen
Recombinant Mouse E-Cadherin/CDH1/E-cad/CD324 Protein (Met1-Val709)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
This antibody has specificity for Mouse E-Cadherin/CDH1/E-cad/CD324.
Target Information
E-Cadherin (epithelial cadherin) is a classical cadherin from the cadherin (alcium dependent adhesion protein) superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. E-cadherin plays a central role in the growth and development of cells by controlling tissue architecture, and maintenance of tissue integrity. In humans, E-cadherin is encoded by the CDH1 gene present on chromosome 16. Studies have demonstrated that reduction and/or loss of E-cadherin expression in carcinomas correlates positively with the potential of these tumors for invasion and metastasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cadherin e; cadherin-E; E-cadherin; Ecadherin; epithelial cadherin; unnamed protein product; uvomorulin
Gene Aliases: ARC-1; E-cad; Ecad; L-CAM; Um; UVO
UniProt ID: (Mouse) P09803
Entrez Gene ID: (Mouse) 12550
calcium ion binding
protein binding
beta-catenin binding
cytoskeletal protein binding
protein phosphatase binding
protein domain specific binding
ankyrin binding
GTPase activating protein binding
identical protein binding
alpha-catenin binding
gamma-catenin binding
cadherin binding
cell adhesion molecule binding
binding, bridging
cell adhesion mediator activity
protein binding involved in cell-cell adhesion
protein tyrosine kinase binding
cell morphogenesis
in utero embryonic development
trophectodermal cell differentiation
desmosome assembly
epithelial cell morphogenesis
cell-cell junction assembly
cell adhesion
homophilic cell adhesion via plasma membrane adhesion molecules
synapse assembly
embryo implantation
sensory perception of sound
regulation of gene expression
negative regulation of protein processing
calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules
cell migration
negative regulation of cell-cell adhesion
positive regulation of cell-cell adhesion
actin cytoskeleton organization
negative regulation of cell migration
negative regulation of axon extension
regulation of protein localization
cell junction assembly
adherens junction organization
uterine epithelium development
positive regulation of protein import into nucleus
cell-cell adhesion mediated by cadherin
positive regulation of transcription, DNA-templated
decidualization
negative regulation of epithelial cell proliferation
intestinal epithelial cell development
salivary gland cavitation
regulation of branching involved in salivary gland morphogenesis
establishment of skin barrier
bicellular tight junction assembly
cellular response to amino acid stimulus
cellular response to lithium ion
cellular response to indole-3-methanol
protein localization to plasma membrane
negative regulation of canonical Wnt signaling pathway
cochlea development
cell-cell adhesion
regulation of protein catabolic process at postsynapse, modulating synaptic transmission
negative regulation of protein localization to plasma membrane
positive regulation of cellular protein localization
regulation of protein localization to cell surface
regulation of neuron migration
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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