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SHANK1/SHANK3 Monoclonal Antibody (N367/51), PerCP

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SHANK1/SHANK3 Monoclonal Antibody (N367/51), PerCP

Product Details

MA5-45675

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (IHC)

Assay-dependent
-

Immunocytochemistry (ICC/IF)

1:100
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

N367/51

Immunogen

Fusion protein amino acids 538-626 (SH3 domain) of rat SHANK3.
3D Epitope / Immunogen

Conjugate

PerCP PerCP PerCP

Excitation/Emission Max

482/675 nm View spectra spectra

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Protein G

Storage buffer

95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA

Contains

no preservative

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2932129

Product Specific Information

Mouse: 100% identity (89/89 amino acids identical). Human: 97% identity (87/89 amino acids identical). ~70% identity with SHANK1 and SHANK2.

1 µg/mL of MA5-45675 was sufficient for detection of SHANK1/SHANK3 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 190kDa. Cross-reacts with SHANK1. Does not cross-react with SHANK2.

This antibody was formerly sold as clone S367-51.

Target Information

Major scaffold postsynaptic density protein which interacts with multiple proteins and complexes to orchestrate the dendritic spine and synapse formation, maturation and maintenance. Interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and HOMER, respectively, and the actin-based cytoskeleton. Plays a role in the structural and functional organization of the dendritic spine and synaptic junction through the interaction with Arp2/3 and WAVE1 complex as well as the promotion of the F-actin clusters. By way of this control of actin dynamics, participates in the regulation of developing neurons growth cone motility and the NMDA receptor-signaling. Also modulates GRIA1 exocytosis and GRM5/MGLUR5 expression and signaling to control the AMPA and metabotropic glutamate receptor-mediated synaptic transmission and plasticity. May be required at an early stage of synapse formation and be inhibited by IGF1 to promote synapse maturation. {PubMed:21606927, PubMed:21795692, PubMed:23100419, PubMed:23897824, PubMed:24089484}.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: brain specific protein; brain-specific protein; GKAP/SAPAP-interacting protein; multidomain postsynaptic density protein; postsynaptic density protein; postsynaptic scaffold protein; proline rich synapse associated protein 2; proline-rich synapse-associated protein 2; SAPAP-interacting protein synamon; SH3/ankyrin domain gene 1; SH3/ankyrin domain gene 3; shank postsynaptic density protein; Shank postsynaptic density protein 3a; shank3 postsynaptic density protein; somatostatin receptor-interacting protein; SPANK-1; SPANK-2; proSAP2; proline-rich synapse-associated protein 2; SSTR-interacting protein; synamon

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Gene Aliases: DEL22q13.3; PROSAP2; PSAP2; SCZD15; Shank1a; SPANK-1; SPANK-2; Spank1; SSTRIP; synamon

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UniProt ID: (Human) Q9Y566, (Human) Q9BYB0, (Rat) Q9WV48, (Mouse) D3YZU1, (Rat) Q9JLU4, (Mouse) Q4ACU6

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Entrez Gene ID: (Human) 50944, (Human) 85358, (Rat) 78957, (Mouse) 243961, (Rat) 59312, (Mouse) 58234

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Function(s)
G-protein coupled receptor binding protein binding SH3 domain binding receptor signaling complex scaffold activity GKAP/Homer scaffold activity somatostatin receptor binding ionotropic glutamate receptor binding identical protein binding macromolecular complex binding ankyrin repeat binding scaffold protein binding structural constituent of postsynaptic density zinc ion binding receptor binding
Process(es)
long-term memory associative learning adult behavior negative regulation of actin filament bundle assembly social behavior protein localization to synapse olfactory behavior habituation modulation of synaptic transmission synapse organization neuromuscular process controlling balance determination of affect righting reflex synapse maturation dendritic spine morphogenesis positive regulation of dendritic spine development protein-containing complex assembly vocalization behavior AMPA selective glutamate receptor signaling pathway maintenance of postsynaptic density structure positive regulation of excitatory postsynaptic potential MAPK cascade embryonic epithelial tube formation synapse assembly learning or memory learning memory locomotory behavior gene expression glial cell proliferation striatal medium spiny neuron differentiation exploration behavior locomotory exploration behavior locomotion vocal learning negative regulation of cell volume regulation of synaptic plasticity positive regulation of long-term neuronal synaptic plasticity brain morphogenesis sensory perception of touch positive regulation of synapse structural plasticity positive regulation of synaptic transmission, glutamatergic long-term synaptic potentiation long term synaptic depression regulation of dendritic spine morphogenesis neural precursor cell proliferation postsynaptic density assembly AMPA glutamate receptor clustering NMDA glutamate receptor clustering guanylate kinase-associated protein clustering response to interleukin-17 regulation of postsynapse organization regulation of long-term synaptic potentiation positive regulation of long-term synaptic potentiation positive regulation of glutamate receptor signaling pathway regulation of long term synaptic depression regulation of AMPA glutamate receptor clustering regulation of grooming behavior regulation of behavioral fear response cognition
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