Invitrogen™

NuPAGE™ LDS Sample Buffer (4X)

Catalog number: NP0008
Invitrogen™

NuPAGE™ LDS Sample Buffer (4X)

Catalog number: NP0008
Catalog Number
NP0008
Unit Size
250 mL
Price (USD)
Price: 169.00
Online price: 161.65
Your price:
Availability
-
Quantity
Catalog Number
Unit Size
10 mL
Price (USD)
Price: 21.38
Online price: 20.65
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
NP0008250 mL
Price: 169.00
Online price: 161.65
Your price:
-
NP000710 mL
Price: 21.38
Online price: 20.65
Your price:
-
Product Overview
Figures
Videos
Recommendations
Recommendations
Documents
FAQ
Citations & References
Additional Information
Recommendations
NuPAGE LDS Sample Buffer (4X) is used to prepare protein samples for denaturing gel electrophoresis with Bis-Tris or Tris-Acetate gels. It contains lithium dodecyl sulfate, pH 8.4, which allows for maximum activity of the reducing agent.

See all available buffers and reagents available for SDS-PAGE

NuPAGE LDS Sample Buffer contains Coomassie G250 and Phenol Red as tracking dyes instead of bromophenol blue. Coomassie G250 gives a sharp dye front with both MES and MOPS SDS running buffers and migrates much closer to the moving ion front than bromophenol blue. Bromophenol blue runs more slowly than some peptides with MES SDS Running Buffer. This ensures that small peptides do not run off the gels.

To use: Heat the sample in a 1X dilution (reduced or non-reduced) at 70°C for 10 minutes for optimal results.

Note: NuPAGE LDS Sample Buffer should be brought to room temperature (25°C) prior to use. It is a highly viscous and concentrated solution containing twice the amount of LDS compared to the amount of SDS in typical sample buffers. NuPAGE LDS Sample Buffer also contains a higher concentration glycerol.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Buffer
Sample Loading Buffers
Concentration
4X
Gel Type
NuPAGE
Product Type
LDS Sample Buffer
Gel Compatibility
NuPAGE™ Gels
Product Line
NuPAGE™
Quantity
250mL
Shipping Condition
Approved for shipment at Room Temperature or on Wet Ice

Contents & Storage

Sample Buffer (4X) containing lithium dodecyl sulfate (LDS) at pH 8.5 with SERVA Blue G250 and phenol red.

Store at 4°C to 25°C.

Figures

Documents & Downloads

Certificates

    Frequently asked questions (FAQs)

    Citations & References

    Search citations by name, author, journal title or abstract text