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Invitrogen
KMT2D Polyclonal Antibody
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FIGURE: 1 / 7
KMT2D Antibody (PA5-57490) in ICC/IF
Immunofluorescence analysis of Histone-lysine N-methyltransferase 2D was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with KMT2D Polyclonal Antibody (Product # PA5-57490) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Ale... View More
Product Details
PA5-57490
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human KMT2D. Recombinant protein control fragment (Product #RP-102441).
Conjugate
Form
Liquid
Concentration
0.10 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: AQLADTLFSK GLGPWDPPDN LAETQKPEQS SLVPGHLDQV NGQVVPEASQ LSIKQEPREE PCALGAQSVK REANGEPIGA PGTSNHLLLA GPRSEAGHLL LQKLLRAKNV QLSTGRGSEG LRAEINGHI
Highest antigen sequence identity to the following orthologs: Mouse - 90%, Rat - 84%.
Target Information
KMT2D is a histone methyltransferase that methylates Lys-4 of histone H3 (H3K4me). H3K4me represents a specific tag for epigenetic transcriptional activation. KMT2D acts as a coactivator for estrogen receptors by being recruited by ESR1 and activating transcription. KMT2D is involved in chromatin organization, chormatin silencig, oocyte growth, oogenesis, and a positive regulation of cell proliferation. Mutations in the KMT2D gene cause Kabuki syndrome (KABUK1) which results in congential mental retardation syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: AAD10; ALL1-related protein; alternatively spliced; similarity to ALL-1 and Drosophila trithorax; Histone-lysine N-methyltransferase 2D; histone-lysine N-methyltransferase MLL2; Kabuki make-up syndrome; lysine (K)-specific methyltransferase 2D; Lysine N-methyltransferase 2D; myeloid/lymphoid or mixed-lineage leukemia 2; Myeloid/lymphoid or mixed-lineage leukemia protein 2; trinucleotide repeat containing 21
Gene Aliases: AAD10; ALR; BCAHH; CAGL114; KABUK1; KMS; KMT2D; MLL2; MLL4; TNRC21
UniProt ID: (Human) O14686
Entrez Gene ID: (Human) 8085
transcription regulatory region sequence-specific DNA binding
DNA binding
transcription coactivator activity
protein binding
methyltransferase activity
zinc ion binding
transferase activity
histone methyltransferase activity (H3-K4 specific)
metal ion binding
histone H3K4 monomethyltransferase activity
histone H3K4 trimethyltransferase activity
oocyte growth
chromatin organization
regulation of transcription, DNA-templated
positive regulation of cell proliferation
heterochromatin assembly
methylation
positive regulation of intracellular estrogen receptor signaling pathway
response to estrogen
positive regulation of transcription from RNA polymerase II promoter
oogenesis
beta-catenin-TCF complex assembly
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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