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Invitrogen
NEIL1 Polyclonal Antibody
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FIGURE: 1 / 2
NEIL1 Antibody (PA5-96438) in WB
Western blot analysis of NEIL1 in extracts of various cell lines. Samples were incubated with NEIL1 Polyclonal antibody (Product # PA5-96438) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-96438
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-135 of human NEIL1 (NP_0788842)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.58 mg/mL
Amount
58 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MPEGPELHLA SQFVNEACRA LVFGGCVEKS SVSRNPEVPF ESSAYRISAS ARGKELRLIL SPLPGAQPQQ EPLALVFRFG MSGSFQLVPR EELPRHAHLR FYTAPPGPRL ALCFVDIRRF GRWDLGGKWQ PGRGP
Positive Samples: Cellular Location: Chromosome, Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center
Target Information
NEIL1 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA endonuclease eight-like glycosylase 1; DNA glycosylase/AP lyase Neil1; DNA-(apurinic or apyrimidinic site) lyase Neil1; endonuclease VIII; endonuclease VIII-like 1; FLJ22402; NEH1; nei endonuclease VIII-like 1; nei homolog 1; nei-like protein 1; unnamed protein product
Gene Aliases: 2810450N13Rik; FPG1; hFPG1; NEH1; NEI1
UniProt ID: (Human) Q96FI4, (Mouse) Q8K4Q6
Entrez Gene ID: (Human) 79661, (Rat) 367090, (Mouse) 72774
nucleic acid binding
damaged DNA binding
DNA-(apurinic or apyrimidinic site) lyase activity
protein binding
zinc ion binding
hydrolase activity, acting on glycosyl bonds
hydrolase activity, hydrolyzing N-glycosyl compounds
lyase activity
DNA N-glycosylase activity
class I DNA-(apurinic or apyrimidinic site) endonuclease activity
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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