Thermo Scientific™

Pierce™ Premium Grade EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride)

Catalog number:  PG82079

Related applications:

Protein Labeling & Crosslinking

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Thermo Scientific Pierce Premium Grade EDC is our highest quality formulation of this popular carbodiimide crosslinker, specially characterized for applications where product integrity and risk minimization are paramount.

Features of Premium Grade EDC:

EDC—carbodiimide crosslinker for conjugation between carboxylates and amines
High quality—identity and purity confirmed by several tests
Product integrity—enhanced level of testing and characterization compared to standard grade
Lot retention—ample supply of past lots retained to ensure future process testing
Change management—Change Control Notification (CCN) service
Consistent manufacture—batch-specific manufacturing documentation review

Compared to the standard grade product, Premium Grade EDC provides more clearly defined quality and product support by including (a) increased analytical testing and product characterization, (b) greater batch-specific information and quality assurance review, (c) extensive lot sample retention and (d) change control notification. EDC is 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride, a water-soluble carbodiimide crosslinker that activates carboxyl groups for spontaneous reaction with primary amines, enabling peptide immobilization and hapten-carrier protein conjugation. In the presence of N-hydroxysulfosuccinimide (sulfo-NHS), EDC can be used to convert carboxyl groups to amine-reactive sulfo-NHS esters. This is accomplished by mixing the EDC with a carboxyl containing molecule and adding sulfo-NHS.

Specifications of Premium Grade EDC
We manufacture crosslinking reagents to ensure the highest possible overall product integrity, consistency and performance for the intended research applications.
• Visual: White crystalline powder, free of foreign matter
• Identity: IR scan shows only peaks characteristic of EDC
• Water content: Karl Fischer titration, ≤ 2%
• Trace metals: All metals, including lead, ≤ 50 ppm
• Elemental analysis: Reported values for C, H, and N

• Conjugate carboxyl and amino groups among peptides and proteins
• Couple haptens to immunogenic carrier proteins (e.g., attach a peptide to KLH)
Immobilize peptide antigens to affinity-purify antibodies
• Create NHS-activated, amine-reactive labeling compounds
• Crosslink proteins to carboxyl coated beads or surfaces
• Activate nanoparticles with amine-reactive sulfo-NHS esters

Product References:
Crosslinker Application Guide -- search for recent literature references for this product

Related Products
EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride)
Pierce™ Premium Grade Sulfo-NHS (N-hydroxysulfosuccinimide)
For Research Use Only. Not for use in diagnostic procedures.


Product Line: Pierce™
Crosslinker Type: Heterobifunctional
Labeling Method: Chemical Labeling
Reactive Moiety: Carbodiimide
Solubility: Water
Spacer: Short

Contents & storage

Store desiccated at -20°C. Shipped with wet ice.