pUC57 DNA

Catalog number: SD0171

Thermo Scientific™  Related applications: Nucleic Acid Gel Electrophoresis & Blotting | Restriction Enzyme Cloning

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Description

Thermo Scientific Plasmid pUC57, 2710 bp in length, is a derivative of pUC19. pUC57 MCS contains 6 restriction sites with protruding 3’-ends that are resistant to E. coli exonuclease III. This vector is designed for cloning and generation of ExoIII deletions. The exact positions of the genetic elements are shown on the map (termination codons included). DNA replication initiates at position 890 (±1) and proceeds in the direction indicated. The bla gene nucleotides 2510-2442 (compl. strand) code for a signal peptide.

Highlights

• More than 90% in the supercoiled form
• Isolated from E. coli (dam+, dcm+)
• Purified by chromatography using proprietary patented technology

Applications

• Cloning
• Sequencing of insert DNA

Related Products
pUC18 DNA
pUC19 DNA
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Vector Type: pUC57
Product Size: 50 µg
Cloning Method: Restriction Enzyme ⁄ MCS

Documents

Manuals & protocols

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