Kit de inicio iBind™ Western

El kit básico iBind Western incluye un dispositivo iBind Western, así como todos los consumibles y reactivos necesarios para empezarMás información

Número de catálogo	Cantidad
SLF1000S	1 kit

Número de catálogo SLF1000S

Precio (MXN)

Cantidad:

1 kit

El kit básico iBind Western incluye un dispositivo iBind Western, así como todos los consumibles y reactivos necesarios para empezar a utilizar este dispositivo de procesamiento automático Western. El dispositivo occidental iBind realiza todos los pasos del protocolo Western, desde el bloqueo hasta los lavados, además de las incubaciones con anticuerpo, mediante un flujo lateral secuencial (SLF). El SLF permite la liberación y flujo oportunos de soluciones y anticuerpos a la membrana sin necesidad de una fuente de alimentación externa, agitadores o bandejas.

Más información sobre los sistemas iBind Western ›

El dispositivo iBind Western funciona exclusivamente con las tarjetas iBind y el kit de soluciones iBind para procesar una minimembrana para inmunodetección. El dispositivo iBind proporciona comodidad automatizada y una reproducibilidad superior a los métodos manuales tradicionales.. Cada solución se libera desde los pocillos del dispositivo iBind hacia una tarjeta iBind a través del SLF en ∼ 2,5 horas. La matriz de fibra de vidrio de la tarjeta permite el flujo uniforme y homogéneo de las soluciones sobre la membrana, lo que aumenta la interacción entre el antígeno y el anticuerpo.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Capacidad1 miniblot (8 x 8 cm)

Cantidad1 kit

Tiempo de actividad∼ 2,5 horas

Método de detecciónCromogénico, quimioluminiscente, fluorescente

Membrane CompatibilityNitrocelulosa, PVDF

Línea de productosiBind

Tipo de productoKit de inicio

Unit Size1 kit

Contenido y almacenamiento

• 1 dispositivo iBind™ WesterniBind
• 10 tarjetas iBind™ (almacenar a temperatura ambiente)
• 1 kit de solución iBind™ (almacenar a 2–8 °C)

Preguntas frecuentes

Do you offer additional years of service for the iBind Western Device?

No, we do not provide service contracts or extended warranties for these devices. The warranty is limited to 1 year from the date of shipment.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What procedure should I follow when stripping and reprobing my blot processed using the iBind Western Device or iBind Flex Western Device?

To strip and reprobe your blot processed using the iBind Western Device or iBind Flex Western Device, first strip the blot outside the system using your preferred protocol. After the stripping protocol, wash the blot in distilled water for 5 min. Reblocking is unnecessary as the iBind Solution will perform this step during reprobing. Finally, reprobe with new antibodies using the iBind Western Device or iBind Flex Western Device, starting with the membrane preparation step.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I multiplex antibodies using the iBind Western Device or iBind Flex Western Device?

Yes, for each midi blot, mini blot, or vertically cut strip, prepare appropriate primary antibodies together in a single tube of iBind FD/ iBind Flex FD Solution, Prepare appropriate secondary antibodies together in a different tube of iBind FD/ iBind Flex FD Solution. Do not mix primary and secondary antibodies prior to loading in the device. All primary and secondary antibodies should be diluted according to the recommendations outlined in the user manual or quick reference card. When detecting several targets of the same species, the same concentration of secondary antibody can be used as when detecting one target. There is no need to increase the concentration for each additional target. Load multiplexed antibodies into the device as normal.
Caution: Consider cross-reactivity of secondary antibodies when multiplexing (e.g., rabbit anti-goat IgG and goat anti-mouse IgG are likely to cross-react).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I use the iBind Western Device or iBind Flex Western Device with Alexa Fluor Plus conjugated secondary antibodies?

Yes, both the iBind Western Device and iBind Flex Western Device work well with Alexa Fluor Plus conjugated secondary antibodies.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

With the iBind Western Device and iBind Flex Western Device, how do I know if I am rolling the membrane firmly enough?

When firmly rolling, the iBind/iBind Flex Card will slightly dip in the membrane area, causing the top and bottom of the card to lift. See the iBind/iBind Flex user manual for additional details.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

View all Kit de inicio iBind™ Western FAQs