Recombinant human BMP-4 (Product # PHC9531) stimulates dose-dependent induction of alkaline phosphatase production in the ATDC-5 mouse chondrogenic cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. ATDC-5 cells were treated with increasing concentrations of recombinant human BMP-4 for 72 hrs hours before lysis and addition of pNPP. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 values r... View More

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Product Details

PHC9531

Applications

Tested Dilution

Publications

Bioactivity (Bioactivity)

Assay-dependent

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Product Specifications

Species

Human, Mouse

Published species

Human, Mouse

Expression System

HEK293 cells

Molecular weight

34 kDa

Class

Recombinant

Type

Protein

Purity

>95% by SDS-PAGE

Endotoxin concentration

<1 EU/µg

Activity

ED50 = 2 - 10 ng/mL; determined by the dose-dependent induction of alkaline phosphatase production in ATDC-5 cells.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Amount

100 µg

Purification

purified

Storage buffer

HCl

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Dry ice

Product Specific Information

Carrier-Free

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute lyophilized recombinant human BMP-4 in 4 mM HCl containing 0.1% human serum albumin. Further dilutions should be made in low endotoxin medium or a buffered solution containing a carrier protein such as heat inactivated FCS or tissue culture grade BSA.

Storage: Store this lyophilized preparation at ≤-20°C, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at ≤-20°C. Avoid repeated freeze/thaw cycles.

Target Information

The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. This particular family member plays an important role in the onset of endochondral bone formation in humans, and a reduction in expression has been associated with a variety of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva. Alternative splicing in the 5' untranslated region of this gene has been described and three variants are described, all encoding an identical protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: BMP; BMP-2B; BMP-4; Bone morphogenetic protein; Bone morphogenetic protein 2B; Bone morphogenetic protein 4; unnamed protein product

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Gene Aliases: Bmp-4; BMP2B; Bmp2b-1; BMP2B1; BMP4; Dvr-4; DVR4; MCOPS6; OFC11; ZYME

Human BMP-4 Recombinant Protein

Human BMP-4 Protein (PHC9531) in Bioactivity