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Product Details
PHG0204
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
Human FGF-10 recombinant protein contains 170 amino acids
Molecular weight
19.3 kDa
Class
Recombinant
Type
Protein
Purity
≥95% by SDS-PAGE and HPLC
Endotoxin concentration
<0.1 ng/µg
Activity
ED50 = 0.5 - 15 ng/mL; determined by the dose-dependent proliferation of 4MBr-5 cells.
Conjugate
Form
Lyophilized
Purification
purified
Storage buffer
5mM sodium phosphate with 80mM NaCl
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Wet or Dry ice (international)
Product Specific Information
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 5 mM sodium phosphate, pH 7.4, to a concentration of 0.1-1.0 mg/mL. Further dilutions should be made in aqueous buffers supplemented with carrier protein, such as 0.1-1.0% BSA
Storage: The lyophilized protein is stable at room temperature for up to 1 month, but should be kept at -20°C for long term storage. Working aliquots stored with carrier protein are stable for at least 3 months at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
Target Information
FGF10 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein exhibits mitogenic activity for keratinizing epidermal cells, but essentially no activity for fibroblasts, which is similar to the biological activity of FGF7. Studies of the mouse homolog of suggested that this gene is required for embryonic epidermal morphogenesis including brain development, lung morphogenesis, and initiation of lim bud formation. This protein is also implicated to be a primary factor in the process of wound healing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FGF; FGF-10; Fibroblast growth factor; Fibroblast growth factor 10; Keratinocyte growth factor 2; produced by fibroblasts of urinary bladder lamina propria
Gene Aliases: FGF10
UniProt ID: (Human) O15520
Entrez Gene ID: (Human) 2255
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