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Product Details
RP-75584
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
Amino acids Ala117-Ser269 containing an N-terminal His-tag
Tag
His-tag
Molecular weight
21.88 kDa
Class
Recombinant
Type
Protein
Purity
>95%, by SDS-PAGE under reducing conditions
Conjugate
Form
Liquid
Concentration
0.05 mg/mL
Purification
purified
Storage buffer
Laemmli, pH 6.8
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Wet ice
Product Specific Information
RP-75584 contains purified recombinant human IL-1 beta protein with an N-terminal hexahistidine tag.
RP-75584 may be used as a positive control in many immunological assays.
Target Information
Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Catabolin; Hematopoietin 1 (H1); IFN beta inducing factor; il 1b; IL 1β; IL-1 beta; IL1 B; IL1β; ILN; Interleukin; Interleukin 1 beta precursor; interleukin 1, beta; Interleukin-1 beta; Interleukin-1b; Interleukin1 beta; LAF; Osteoclast activating factor (OAF); preinterleukin 1 beta; Pro interleukin 1 beta; pro-interleukin-1-beta
Gene Aliases: IL-1; IL1-BETA; IL1B; IL1F2
UniProt ID: (Human) P01584
Entrez Gene ID: (Human) 3553
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