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Product Details
PHG0041
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIEIVRK KPIFKKATVT LEDHLACKCE TVAAARPVT
Molecular weight
25.4 kDa
Class
Recombinant
Type
Protein
Purity
>95% by SDS-PAGE
Endotoxin concentration
<0.1 ng/µg
Activity
ED50 ≤ 5 ng/mL; determined by the dose-dependent proliferation of BALB/c 3T3 cells.
Conjugate
Form
Lyophilized
Purification
purified
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Wet ice
Product Specific Information
Carrier-Free
Reconstitution: Centrifuge the vial briefly, before opening to bring the contents to the bottom. Reconstitute the lyophilized protein in 100mM acetic acid containing 0.1% BSA to 0.1-1.0 mg/mL to regain full activity. Apportion the reconstituted protein into working aliquots and store at ≤-20°C. Make any further dilutions of the reconstituted protein in low endotoxin medium or buffered solution with FBS or tissue culture grade BSA.
Storage: Store the lyophilized protein at 2-8°C, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at ≤-20°C. Avoid repeated freeze-thaw cycles.
Target Information
The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two alternatively spliced transcript variants encoding different isoforms have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: becaplermin; FLJ12858; H-PDGF-BB; PDGF subunit B; PDGF, B chain; PDGF-2; platelet-derived growth factor 2; Platelet-derived growth factor B chain; Platelet-derived growth factor beta polypeptide; platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog); Platelet-derived growth factor subunit B; platelet-derived growth factor, beta polypeptide (oncogene SIS); Proto-oncogene c-Sis
Gene Aliases: c-sis; IBGC5; PDGF-2; PDGF2; PDGFB; SIS; SSV
UniProt ID: (Human) P01127
Entrez Gene ID: (Human) 5155
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