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Gibco
PeproGMP® Human EGF Recombinant Protein, PeproTech®
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PeproGMP® Human EGF Protein (GMP100-15-100UG) in Bioactivity
Cell proliferation assay analysis of PeproGMP® Human EGF Recombinant Protein, PeproTech (Product # GMP100-15-100UG) compared to Human EGF, Animal-Free Recombinant Protein, PeproTech (Product # AF-100-15-1MG), demonstrating comparable bioactivity across product grades.
Product Details
GMP100-15-100UG
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Published species
Human,
Mouse
Expression System
E. coli
Amino acid sequence
NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR
Molecular weight
6.2 kDa
Class
Recombinant
Type
Protein
Purity
≥ 98% by SDS-PAGE gel and HPLC analyses.
Endotoxin concentration
≤0.1 EU/µg
Activity
Determined by the proliferation of mouse Balb/c 3T3 cells. PeproGMP Recombinant Human EGF has been tested against the WHO standard NIBSC 91/530. Find the International Unit (IU) value here.
Conjugate
Form
Lyophilized
Purification
purified
Contains
no preservative
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient
Product Specific Information
The EGF protein is a potent growth factor that stimulates the proliferation of various epiderminal and epithelial cells. The PeproTech Recombinant Human EGF is a 6.2 kDa globular protein containing 53 amino acid residues including 3 intramolecular disulfide bonds.
Reconstitute using sterile water (i.e., WFI) to a concentration of 0.1-1.0 mg/ml.
Additional product information, specifications, handling and reconstitution information are provided on the Certificate of Analysis.
All PeproGMP® products are manufactured using animal-derived component free materials.
Product Description/Intended Use: PeproTech GMP recombinant proteins are manufactured for use as ancillary materials by applying applicable principles of GMP and quality control requirements from USP (United States Pharmacopeia) Chapter <1043> Ancillary Materials for Cell, Gene, and Tissue-Engineered Products.
Target Information
EGF (Epidermal growth factor) exerts its actions by binding to the EGF Receptor, a 170 kDa protein kinase. Activation of EGFR initiates diverse cellular pathways in response to toxic environmental stimuli, or to EGF binding to the receptor, the EGFR forms homo- or heterodimers with other family members. Each dimeric receptor complex initiates a distinct signaling pathway by recruiting different Src homology 2 (SH2) containing effector proteins. EGF is far and wide expressed in kidney, cerebrum, prostrate and salivary glands. EGF acts as a potent mitogenic factor and the phosphorylated receptor recruits adapter proteins like GRB2 that activates complex downstream signaling cascades. EGF activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STAT modules. Research studies suggest the protein may also play important role in activating the NF-kappa-B signaling cascade. Defects in the EGGF gene are the cause of hypomagnesemia type 4 and dysregulation has been associated with the growth and progression of certain cancers.
For research use or further manufacturing. Not for diagnostic use or direct administration into humans or animals.
Bioinformatics
Protein Aliases: beta-urogastrone; EGF; Epidermal growth factor; H-EGF; Pro-epidermal growth factor
Gene Aliases: EGF; HOMG4; URG
UniProt ID: (Human) P01133
Entrez Gene ID: (Human) 1950
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