QuantStudio12K

Get more answers with our flexible-content real-time PCR solution

Respiratory tract infections can lead to a number of diseases of the upper and lower respiratory tracts that are caused by microbes such as viruses, bacteria, and even fungi. Detection of these pathogens can be challenging; immunoassays provide limited answers, and culture based methods are prone to contamination and can miss bacterial and fungal pathogens owing to fastidious growth in culture. Concurrent prevalence of viral and bacterial pathogens is a growing concern and needs effective detection options.

While molecular testing is more sensitive, most commercially available molecular tests are expensive, primarily focus on either viruses or bacteria, and lack the flexibility to include specific targets organisms that are clinically relevant to a patient segment or regional population.

Our real-time PCR solution for respiratory tract microbiota (RTM) is a sensitive, syndromic panel–based, flexible-content solution for the detection of respiratory pathogens. The Applied Biosystems TaqMan Array Respiratory Tract Microbiota Comprehensive Card combines the power of the QuantStudio 7 and 12K Flex real-time PCR systems with TaqMan Assays.

Features of this solution include:

  • Extensive coverage—with 42 validated pathogen targets, our solution features the most pathogenic markers of any test on the market
  • Customizable—our solution allows you to develop customized, relevant panels to match your specific population and testing needs
  • Cost-effective—lowest cost per sample when compared with other commercially available molecular solutions
  • Multiple sample input types—including nasopharyngeal swabs, bronchoalveolar lavage (BAL), and nasal nasopharyngeal aspirate

 

What’s included as part of this qualified solution?

Qualified TaqMan Assays 

TaqMan Array Card: Respiratory Tract Microbiota Card v4

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TaqMan PreAmp Pool, Respiratory Tract Microbiota

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Controls

TaqMan Universal DNA Spike-in Control

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TaqMan Respiratory Tract Microbiota Amplification Control

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Data and support

Application note 

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Workflow application guide 

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Compliance and training products/services 

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Explore respiratory pathogen targets included in our solution

TaqMan array card for respiratory tract microbiota

No. of wells 384
No. of targets 42 respiratory plus 4 control (Xeno, 18S, RNase P, and Bacillus atropheus)
No. of samples 8
Master mix volume 400 μL per TaqMan array card (384 reactions)
Reaction volume 1 μL
Samples/day Up to 64
Compatible instruments QuantStudio 12K Flex Real-TimePCR System with TaqMan array card block 
Time-to-results 5 hours

42 verified TaqMan Assays are preloaded on the RTM TaqMan array card

Pathogen type Target pathogen
Bacterial Bordetella
Bordetella holmesii
Bordetella pertussis
Chlamydophila pneumoniae
Mycoplasma pneumoniae
Streptococcus pneumoniae
Coxiella burnetii
Staphylococcus aureus
Klebsiella pneumoniae
Legionella pneumophila
Moraxella catarrhalis
Haemophilus influenzae
Fungal Pneumocystis jirovecii

42 verified TaqMan Assays are preloaded on the RTM TaqMan array card (continued)

Pathogen type Target pathogen
Viral Adenovirus
Coronavirus HKU1
Coronavirus NL63
Coronavirus 229E
Coronavirus OC43
Human metapneumovirus
Rhinovirus
Enterovirus
Enterovirus D68
Influenza A (Pan)
Influenza A/H1-2009
Influenza A/H3
Influenza B
Parainfluenza 1
Parainfluenza 2
Parainfluenza 3
Parainfluenza 4
Respiratory Syncytial Virus A
Respiratory Syncytial Virus B
Bocavirus
Epstein-Barr virus (EBV)
SARS-CoV
MERS-CoV
Mumps
Measles
Cytomegalovirus
Human herpesvirus 6 (HHV-6)
Varicella zoster virus (VZV)
Parechovirus