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Get more answers with our flexible-content real-time PCR solution

Respiratory tract infections can lead to a number of diseases of the upper and lower respiratory tracts that are caused by microbes such as viruses, bacteria, and even fungi. Detection of these pathogens can be challenging; immunoassays provide limited answers, and culture based methods are prone to contamination and can miss bacterial and fungal pathogens owing to fastidious growth in culture. Concurrent prevalence of viral and bacterial pathogens is a growing concern and needs effective detection options.

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While molecular testing is more sensitive, most commercially available molecular tests are expensive, primarily focus on either viruses or bacteria, and lack the flexibility to include specific targets organisms that are clinically relevant to a patient segment or regional population.

Our real-time PCR solution for respiratory tract microbiota (RTM) is a sensitive, syndromic panel–based, flexible-content solution for the detection of respiratory pathogens. We have verified solutions using both OpenArray and TaqMan Array Card technology, which you can order as off-the-shelf or customizable, depending on your unique needs.

Features of this solution include:

  • Extensive coverage—with 42 validated pathogen targets, our solution features the most pathogenic markers of any test on the market
  • Customizable—our solution is verified for TaqMan Array Cards and OpenArray plates, along with customized solutions for 96- and 384-well plates, or single tube assays
  • Cost-effective—up to three times more cost effective compared to other commercially available panel-based molecular respiratory tests
  • Multiple sample input types—including nasopharyngeal swabs, bronchoalveolar lavage (BAL), and nasal nasopharyngeal aspirate

What’s included as part of this qualified solution?

Qualified TaqMan Assays 

TaqMan array card: Respiratory Tract Microbiota Comprehensive Card v4

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OpenArray: TaqMan OpenArray Respiratory Tract Microbiota Plate vB

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TaqMan PreAmp Pool, Respiratory Tract Microbiota

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Controls

TaqMan Respiratory Tract Microbiota Amplification Control

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TaqMan Universal RNA Spike In / Reverse Transcription (Xeno) Control

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TaqMan Universal Extraction Control Organism

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Data and support

Application note 

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Workflow application guide 

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Compliance and training products/services 

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Explore respiratory pathogen targets included in our solution

TaqMan array card format for respiratory pathogen detection

No. of wells 384
No. of targets 42 respiratory plus 4 control (Xeno, RNase P, and Bacillus atropheus)
No. of samples 8
Master mix volume 400 μL per TaqMan array card (384 reactions)
Reaction volume 1 μL
Samples/day Up to 64
Compatible instruments QuantStudio 12K Flex Real-TimePCR System with TaqMan array card block
Time-to-results 5 hours

OpenArray plate format for respiratory pathogen detection

No. of wells 2,688
No. of targets 31 respiratory plus 4 control (Xeno, RNase P, and Bacillus atropheus)
No. of samples Up to 24 samples per plate (spotted in triplicate)
Master mix volume 120 μL per OpenArray plate (3,072 reactions)
Reaction volume 33 nL
Samples/day Up to 288
Compatible instruments QuantStudio 12K Flex Real-TimePCR System with OpenArray block
Time-to-results 5 hours

Respiratory tract microbiota targets

All are included with TaqMan array card. An asterisk (*) indicates those included with OpenArray plate.

Pathogen type Target pathogen
Bacterial Bordetella (PAN)*
Bordetella holmesii
Bordetella pertussis*
Chlamydophila pneumoniae*
Mycoplasma pneumoniae*
Streptococcus pneumoniae*
Coxiella burnetii
Staphylococcus aureus
Klebsiella pneumoniae*
Legionella pneumophila*
Moraxella catarrhalis
Haemophilus influenzae*
Fungal Pneumocystis jirovecii
Pathogen type Target pathogen
Viral Adenovirus*
Coronavirus HKU1*
Coronavirus NL63*
Coronavirus 229E*
Coronavirus OC43*
Human metapneumovirus*
Rhinovirus*
Enterovirus*
Enterovirus D68
Influenza A (Pan)*
Influenza A/H1-2009*
Influenza A/H3*
Influenza B*
Parainfluenza 1*
Parainfluenza 2*
Parainfluenza 3*
Parainfluenza 4*
Respiratory Syncytial Virus A*
Respiratory Syncytial Virus B*
Bocavirus*
Epstein-Barr virus (EBV)*
SARS-CoV
MERS-CoV
Mumps
Measles
Cytomegalovirus*
Human herpesvirus 6 (HHV-6)*
Varicella zoster virus (VZV)*
Parechovirus

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White Paper: Using flexible-content syndromic panels to help manage infectious disease: update on laboratory trends and health outcomes

Learn how flexible-content real-time PCR syndromic panels allow you to test for multiple markers at once, making them a more cost-efficient method to get the most from clinically relevant tests.

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