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Each phase has been optimized for a different class of compound. Select the optimal phase for your carbohydrate sample from this family of Thermo Scientific Dionex CarboPac columns.
Column | Recommended use | Target application |
---|---|---|
Dionex CarboPac PA210-Fast-4µm IC Columns | Fast, high-resolution separation of mono-, di-, tri-, tetra-, and pentasaccharides in food and beverage samples without the need for derivatization. Requires high-pressure IC system for fastest runs and optimal operation. | Direct quantification of nonderivatized sugars including glucose, galactose, fructose, sucrose, rhamnose, stachyose, raffinose, and verbascose by IC with pulsed amperometric detection (PAD) |
Dionex CarboPac PA20-Fast-4µm IC Columns | Fast, high-resolution separation of mono- and disaccharides without derivatization, including optimized resolution of glucosamine/galactose and glucose/mannose peak pairs. Requires high-pressure IC system for fastest runs and optimal operation. | Mono- and disaccharide analyses in foods, beverages, glycoprotein therapeutics, and mammalian glycoproteins. |
Dionex CarboPac PA200 IC Columns | Fast, pH-stable and high-resolution mapping and analysis of charged and neutral oligosaccharides with no need for derivatization; separations based on size, charge, degree of branching, and linkage isomerism | Analyses of carbohydrates released from glycoproteins including monosaccharides, disaccharides, oligosaccharides, smaller polysaccharides, sugar acids (such as sialic acids), sugar alcohols, sugar phosphates, and sugar nucleotides (such as N-linked oligosaccharides from glycoprotein therapeutics) |
Dionex CarboPac PA20 IC Columns | High-resolution separations of mono- and disaccharides with no need for derivatization, including optimized resolution of glucosamine/galactose and glucose/mannose peak pairs | Mono- and disaccharide analyses in foods, beverages, glycoprotein therapeutics, and mammalian glycoproteins |
Dionex CarboPac PA20 Fast Sialic Acid IC Column | Fast analysis of sialic acids (e.g., N-acetyl- and N-glycolylneuraminic acids) in glycoprotein acid hydrolyzates with no need for derivatization | Analyses of monosaccharides and sialic acids in glycoproteins, and evaluation of protein glycosylation in limited-quantity samples |
Dionex CarboPac MA1 IC Columns | Analysis of reduced sugars (sugar alcohols) without the need for derivatization | Separation of reduced mono- and disaccharides commonly found in food products (such as commercial sweeteners), glycoproteins, and physiological samples |
Dionex CarboPac SA10 IC Columns | Fast, high-capacity, high-resolution separations of mono- and disaccharides mono- and disaccharides without compromising resolution | Analyses of monosaccharides and disaccharides in biofuels, foods, and beverages |
Dionex CarboPac SA10-4µm IC Columns | Fast, high-resolution, and high-capacity separation of mono- and disaccharides with better resolution and easier peak identification and integration. Requires high-pressure IC system to operate at standard flow rates. | Analyses of monosaccharides and disaccharides in biofuels, foods, and beverages |
Dionex CarboPac SA10-Fast-4µm IC Columns | Fast separation and accurate quantification of mono- and disaccharides with better resolution and easier peak identification and integration. Requires high-pressure IC system for fastest runs and optimal operation. | Separation of simple sugars in biofuels and food samples |
Dionex CarboPac PA100 IC Columns | Low picomole level analysis of without sample derivatization; separation of neutral and charged oligosaccharides in the same run | Analyses of closely related and isomeric oligosaccharides and resolution of oligosaccharides released from therapeutic glycoproteins differing in size, charge, branching, and linkage |
Dionex CarboPac PA10 IC Columns | Isocratic separation of mono- and disaccharides as well as quantification of acidic, neutral, and aminomono-saccharides without derivatization | Separation of mono- and disaccharides in foods, drugs, plants, and mammalian glycoproteins; separation of sialic acids when sodium acetate is added to the eluent |
Dionex CarboPac PA1 IC Columns | Separation of mono- and disaccharides without derivatization, as well as specific oligosaccharides using an isocratic eluent; analysis of sialic acid | Analyses of mono-, disaccharides, oligosaccharides, linear polysaccharides, and aminoglycosides in foods for nutritional labeling using a variety of official methods; separation of neutral and acidic mono- and disaccharides |
High performance anion-exchange chromatography with pulsed ameromentric detection (HPAE-PAD) is a well established method for carbohydrate analysis. Using Dionex CarboPac columns, HPAE-PAD provides high-resolution separations of sugar alcohols, mono-, di-, poly-, and oligosaccharides, sialic acids, and a wide variety of other carbohydrates. We also offer small particle columns for high-pressure ion chromatography systems.