Monitor and improve Salmonella control measures across the food production chain with the Thermo Scientific™ SureCount™ Salmonella species, Typhimurium and Enteritidis Multiplex PCR Kit workflow.

A simple, all-in-one PCR solution designed to give you quantification and serotype results for the most common pathogenic Salmonella strains associated with meat and poultry matrices.

SureCount Multiplex PCR Kit

The new SureCount Multiplex PCR Kit method, which is currently undergoing AOAC Performance Tested Methods (PTM) validation for poultry, beef, and pork matrices, will be the first to enable validated simultaneous quantification and typing of Salmonella species, S. Typhimurium and S. Enteritidis in a single PCR test.

According to the AOAC-PTM validation study, the method is able to quantify 1 CFU per tested poultry rinses, raw meat and poultry samples (chicken carcass rinses, ground beef, ground pork and ground turkey). The method can effectively replace laborious, time- and resource-hungry enumeration methods such as the most probable number (MPN) technique and help to drive productivity up, and costs down, without compromising on safety.




a powerful method to inform your Salmonella control program for poultry, beef and pork 



results for all targets available in around 8 hours 



intuitive sample set-up and automated result interpretation


Workflows for meat and poultry


The Thermo Scientific SureCount Salmonella Multiplex PCR Kit method has recently been evaluated against the FSIS-MLG Chapter 4.11 and Appendix 2.05 reference methods for the isolation, identification and enumeration of Salmonella spp Enteritidis and Typhimurium, adhering to AOAC Appendix J guidelines with validated protocols and automated data analysis for meat (beef & pork) & poultry finished goods (AOAC PTM certification pending).


Custom protocols for in-process poultry carcass rinses and primary production samples are also available.

The SureCount Salmonella Multiplex PCR Kit runs on the Applied Biosystems™ QuantStudio 5 Real-Time Food Safety PCR System with results automatically interpreted into the number of colony-forming units (CFU), using the Thermo Scientific™ RapidFinder™ Analysis Software v2.0 or later.

Watch the workflow video ›


To learn more about the full portfolio of Thermo Scientific™ PCR Assays and workflows for the detection of pathogens and other microbial targets in food visit or request to speak to one of our representatives.

Get in contact




Style Sheet for Global Design System
Style Sheet for Komodo Tabs