Quantitation of Opiates in Urine Using High Resolution Mass Spectrometry
Can opiates in human urine be quantitated both quickly and accurately using high resolution mass spectrometry? With Orbitrap HR/AM mass spectrometry, precision doesn’t need to be sacrificed for speed.
Overview: Forensic toxicologists perform analysis of human urine to search for the presence of illegal and controlled substances, including opiates such as morphine or oxycodone. These analyses can play a part in law enforcement decisions; thus it’s critical that test results be highly precise. With a large number of samples to analyze, it’s also important for the process to be speedy and efficient.
Method: Using a Thermo Scientific Q Exactive Focus Hybrid Quadrupole-Orbitrap Mass Spectrometer, six opiates in human urine samples are quantified down to low ng/mL levels. Opiates include morphine, codeine, hydromorphone, hydrocodone, oxymorphone, and oxycodone. The method precision and accuracy were evaluated by running a calibration curve and quintuplicate replicates of quality controls on three different days.
Table 1. Inter-assay precision and bias.
Results: The Q Exactive Focus MS accurately quantitated all six opiates tested. The average recovery across 58 donor urine samples obtained from a collaborator laboratory ranged from 69% to 81% for the six internal standards evaluated. The Q Exactive Focus MS is a versatile instrument capable of both screening large panels and quantitative confirmation of specific panels.