Create MyQubit .qbt files for the Qubit 3 and 4 Fluorometer

This web tool will help you to create custom MyQubit assay .qbt files that you can upload to your Qubit 3 and 4 Fluorometer. If the assay you're creating does not require calibration, simply enter the name your assay then select the appropriate excitation and emission parameters. For assays that require calibration, we recommend using the “MyQubit EZ Guide” Excel® spreadsheet to help you generate the necessary constants. For complete instructions on how to use the MyQubit EZ Guide to generate constants and how to upload .qbt files to your Qubit 3 and 4 Fluorometer, see MyQubit Custom Assays on the Qubit 3 and 4 Fluorometer.

Please note that this web tool creates .qbt files that can only be used on the Qubit 3 and 4 Fluorometer.
To create .qbt files for the Qubit 2.0 Fluorometer, see MyQubit Custom Assays on the Qubit 2.0 Fluorometer for detailed instructions.

Calibration
Indicates whether you are creating an assay that requires calibration or not.
"YES" indicates that the assay will require calibration. All assays created using
MyQubit that require calibration will utilize two standards, the first of which is always
a sample blank (analyte concentration of "0") and the second of which is a sample of
a concentration to be determined by the user. "NO" indicates that the assay will not
require calibration. In this case, the output will be a raw fluorescence value or values.


Assay Name
Indicates the name visible on the initial home screen,
i.e., the "family" of assays. For example, "DNA" is
an Assay Name on the factory-configured instrument.

Name Sub-text
Indicates any smaller sub-text under the assay name
(or to the right of the assay name from the graphical
display). For example, "High Sensitivity" is a
Name Sub-text on the factory-configured instrument.

Excitation
BLUE = 470 nm, filter 430–495 nm. RED = 635 nm,
filter 600–645 nm.

Emission
BOTH is only acceptable if Calibration is "NO".
GREEN = 510–580 nm. FAR RED = 665–725 nm.

Green RFU Name
Indicates the name given to the
displayed RFU output for Green emission when
Calibration is "NO". Only needed when Calibration is
"NO" and Emission is "GREEN" or "BOTH".

Far Red RFU Name
Indicates the name given to the
displayed RFU output for Far Red emission when
Calibration is "NO". Only needed when Calibration is
"NO" and Emission is "FAR RED" or "BOTH".

Units
Only required when Calibration is "YES". Maximum
length 7 characters.

Std Signal Ratio Limit
Only needed when Calibration is "YES". This number
specifies the amount, in raw fluorescence, that the signal
for Standard #2 must exceed the signal for Standard #1
for your MyQubit assay. This is meant to help troubleshoot
large oversights in calibration. If you are unsure of a
reasonable threshold, or if this is not of concern to you,
then enter a small value that you will be assured of
attaining in most calibrations.

Low
Only needed when Calibration is "YES". Indicates the
lowest sample concentration that the instrument will
display a result for. Samples with concentrations below
this value will be reported as "< Low" and will display the
message "*Sample TOO LOW*".

High
Only needed when Calibration is "YES". Indicates the
highest sample concentration that the instrument will
display a result for. Samples with concentrations above
this value will be reported as "> High" and will display
the message "*Sample TOO HIGH*".

N
Only needed when Calibration is "YES". One of three
algorithm constants that must be determined by the user.
Indicates the linearity of the curve, with a value of 1.00
corresponding to completely linear.

K
Only needed when Calibration is "YES". One of three
algorithm constants that must be determined by the user.
Indicates the portion of the curve to focus on.

S
Only needed when Calibration is "YES". One of three
algorithm constants that must be determined by the user.
Indicates the concentration value of Standard #2 used in
assay calibration.


* Required fields

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