Formulations of mounting media that can add favorable properties such as optimizing the refractive index to match that of glass, preventing photobleaching, or preserving samples for long-term storage are widely available. Keep in mind that hard-setting mountants require time to “cure” or harden.
Try to limit the formation of bubbles in your mounting medium. Some bubbles are inevitable, and you can image around them as long as there are only a few.
To avoid bubbles:
This video explains step-by-step how to mount a coverslip properly for cellular imaging as illustrated in steps 1-4 of the below protocol.
|1||Apply a small amount of mounting medium to the surface of the slide; try to use an amount that will just fill the space under the coverslip.|
|2||Remove coverslip containing the sample from the buffer.|
|3||Blot excess buffer or solvent from the non-sample surface of the coverslip (or allow it to air-dry and then remove the salt residue before imaging).|
|4||Slowly tip the coverslip onto the mounting medium, and avoid creating bubbles as you lower it into place.|
|5||Follow manufacturer’s directions for curing time|
|6||Optional: Seal the coverslip if desired.|
For Research Use Only. Not for use in diagnostic procedures.