NOTE: This map applies only to our new and improved pSilencer 2.1-U6 neo vector which began shipping in Dec 2003 (Lots 113P06 & 113P07).

pSilencer™ 2.1-U6 neo Vector Map

rnai epigenetics gene

This map and the annotated sequence below portrays the circular negative control vector provided with the kit. It includes the negative control siRNA template (66 base pairs) between the BamH I and Hind III sites.


pSilencer 2.1-U6 neo Annotated Promoter/Cloning Site Region

We recommend the following primers for sequencing:

M13F(-40): 5'-GTTTTCCCAGTCACGAC-3'

Rev: 5'-GAGTTAGCTCACTCATTAGGC-3'



 


pSilencer 2.1-U6 neo Restriction Sites

Enzymes with single Restriction Sites

Site Enzyme
4456 AatII
1151 AflIII
2044 AhdI
1567 AlwNI
4008 AvaI
4029 AvrII
730 BamHI
3462 BanII
2549 BcgI
2583 BcgI'
3964 BsaBI
2105 BsaI
1026 BsaXI
996 BsaXI`
4039 BseRI
45 BsmBI
2903 BsmI
436 DraIII
3913 EagI
396 EcoRI
792 HindIII
3741 MscI
3139 NruI
1151 PciI
436 PmlI
2962 PsiI
3744 PstI
3304 RsrII
2524 ScaI
4261 SexAI
4010 SmaI
4034 StuI
3704 Tth111I
4008 XmaI
2643 XmnI
4454 ZraI

Non-Cutting Enzymes:

Enzyme
AarI
Acc65I
AccI
AfeI
AflII
AgeI
AleI
AloI
AloI'
ApaI
AscI
AsiSI
BaeI
BaeI'
BbsI
BbvCI
BglII
BlpI
BmgBI
BmtI
Bpu10I
BsgI
BsiWI
BspEI
BsrGI
BstBI
BstEII
BstXI
BstZ17I
Bsu36I
BtrI
ClaI
EcoICRI
EcoNI
EcoRV
FseI
FspAI
HincII
HpaI
KpnI
MfeI
MluI
NheI
NotI
PacI
PflMI
PmeI
PpuMI
PshAI
PspOMI
PsrI
PsrI'
SacI
SacII
SalI
SanDI
SbfI
SfiI
SgfI
SgrAI
SnaBI
SpeI
SrfI
SwaI
TaqII'
XbaI
XcmI
XhoI