AM5760: pSilencer™ 2.1-U6 hygro
Vector Size: 4885 bp
Promoter: Human U6
Ampicillin resistance gene
Hygromycin resistance gene (SV40 early promoter, SV40 early polyadenylation signal)
ColE1 origin of replication
Vector Map and Related Information
We recommend the following primers for sequencing:
T7 primer: 5'-TAATACGACTCACTATAGGG-3'
2.0rev primer: 5'-AGGCGATTAAGTTGGGTA-3'
Note: This is the sequence of the negative control vector provided with the kit. It contains a negative control siRNA template (66 base pairs) between the BamH I and Hind III sites.