AM5764: pSilencer™ 2.1-U6 neo
 
Features:

NOTE: This sequence is for the new and improved pSilencer 2.1-U6 neo vector which began shipping in Dec 2003.

Please see the New Vector Map for diagram, annotated sequence, and restriction sites of our new vector.

Vector Size: 4521 bp
Promoter: Human U6
Ampicillin resistance gene
Neomycin resistance gene (SV40 early promoter, SV40 early polyadenylation signal)
ColE1 origin of replication
We recommend the following primers for sequencing:
M13F(-40): 5'-GTTTTCCCAGTCACGAC-3'
Rev: 5'-GAGTTAGCTCACTCATTAGGC-3'

Note: This is the sequence of the negative control vector provided with the kit. It contains a negative control siRNA template (66 base pairs) between the BamH I and Hind III sites.

Sequence: